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Human PDGFRA ELISA Kit

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  • Alternative name

    Human PDGF-R-alpha ELISA Kit;Human PDGFR-alpha ELISA Kit;Human Alpha platelet-derived growth factor receptor ELISA Kit;Human alpha-type platelet-derived growth factor receptor ELISA Kit;Human CD140 antigen-like family member A ELISA Kit;Human CD140a antigen ELISA Kit;Human Platelet-derived growth factor alpha receptor ELISA Kit;Human platelet-derived growth factor receptor 2 ELISA Kit;Human PDGFR-2 ELISA Kit;Human PDGFR2 ELISA Kit;Human RHEPDGFRA ELISA Kit;Human CD140A ELISA Kit;Human platelet derived growth factor receptor alpha ELISA Kit;Human platelet-derived growth factor receptor alpha ELISA Kit;Human platelet-derived growth factor receptor, alpha polypeptide ELISA Kit;
  • Catalog
    E029674
  • species
    Human
  • GenePDGFRA
  • Standard CurveHuman PDGFRA ELISA Kit
  • Other Species Mouse Pdgfra ELISA KitChicken PDGFRA ELISA KitRat Pdgfra ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Receptor Alpha (PDGFRa). No significant cross-reactivity or interference between Platelet Derived Growth Factor Receptor Alpha (PDGFRa) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman PDGFRA ELISA Kit allows for the in vitro quantitative determination of PDGFRA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Receptor Alpha (PDGFRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Receptor Alpha (PDGFRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Receptor Alpha (PDGFRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Receptor Alpha (PDGFRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Platelet-derived growth factor receptor alpha Protein information
  • Uniprot ID PGFRA_HUMAN
  • Uniprot AC P16234; B2RE69; E9PBH0; Q6P4H5; Q96KZ7; Q9UD28;
  • UniGene Hs.74615;
  • GeneID 5156
  • KEGG hsa:5156;
  • Human Platelet-derived growth factor receptor alpha Protein SEQUENCE

  • SEQUENCE 1089 AA; 122670 MW; 5E3FB9940ACD1BE8 CRC64;

    MGTSHPAFLV LGCLLTGLSL ILCQLSLPSI LPNENEKVVQ LNSSFSLRCF

    GESEVSWQYP MSEEESSDVE IRNEENNSGL FVTVLEVSSA SAAHTGLYTC

    YYNHTQTEEN ELEGRHIYIY VPDPDVAFVP LGMTDYLVIV EDDDSAIIPC

    RTTDPETPVT LHNSEGVVPA SYDSRQGFNG TFTVGPYICE ATVKGKKFQT

    IPFNVYALKA TSELDLEMEA LKTVYKSGET IVVTCAVFNN EVVDLQWTYP

    GEVKGKGITM LEEIKVPSIK LVYTLTVPEA TVKDSGDYEC AARQATREVK

    EMKKVTISVH EKGFIEIKPT FSQLEAVNLH EVKHFVVEVR AYPPPRISWL

    KNNLTLIENL TEITTDVEKI QEIRYRSKLK LIRAKEEDSG HYTIVAQNED

    AVKSYTFELL TQVPSSILDL VDDHHGSTGG QTVRCTAEGT PLPDIEWMIC

    KDIKKCNNET SWTILANNVS NIITEIHSRD RSTVEGRVTF AKVEETIAVR

    CLAKNLLGAE NRELKLVAPT LRSELTVAAA VLVLLVIVII SLIVLVVIWK

    QKPRYEIRWR VIESISPDGH EYIYVDPMQL PYDSRWEFPR DGLVLGRVLG

    SGAFGKVVEG TAYGLSRSQP VMKVAVKMLK PTARSSEKQA LMSELKIMTH

    LGPHLNIVNL LGACTKSGPI YIITEYCFYG DLVNYLHKNR DSFLSHHPEK

    PKKELDIFGL NPADESTRSY VILSFENNGD YMDMKQADTT QYVPMLERKE

    VSKYSDIQRS LYDRPASYKK KSMLDSEVKN LLSDDNSEGL TLLDLLSFTY

    QVARGMEFLA SKNCVHRDLA ARNVLLAQGK IVKICDFGLA RDIMHDSNYV

    SKGSTFLPVK WMAPESIFDN LYTTLSDVWS YGILLWEIFS LGGTPYPGMM

    VDSTFYNKIK SGYRMAKPDH ATSEVYEIMV KCWNSEPEKR PSFYHLSEIV

    ENLLPGQYKK SYEKIHLDFL KSDHPAVARM RVDSDNAYIG VTYKNEEDKL

    KDWEGGLDEQ RLSADSGYII PLPDIDPVPE EEDLGKRNRH SSQTSEESAI

    ETGSSSSTFI KREDETIEDI DMMDDIGIDS SDLVEDSFL

  • UCSC uc003hal.4; human. [P16234-1];


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