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Human PDGFD ELISA Kit

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  • Alternative name

    Human PDGF-D ELISA Kit;Human iris-expressed growth factor ELISA Kit;Human spinal cord-derived growth factor B ELISA Kit;Human SCDGF-B ELISA Kit;Human PDGFD latent form ELISA Kit;Human PDGFD receptor-binding form ELISA Kit;Human IEGF ELISA Kit;Human SCDGFB ELISA Kit;Human MSTP036 ELISA Kit;Human platelet derived growth factor D ELISA Kit;Human platelet-derived growth factor D ELISA Kit;
  • Catalog
    E029670
  • species
    Human
  • GenePDGFD
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PDGFD ELISA Kit allows for the in vitro quantitative determination of PDGFD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical    Principle of the Assay: PDGFD ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDGFD. Standards or samples are then added to the microtiter plate wells and PDGFD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDGFD present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PDGFD are added to each well to "sandwich" the PDGFD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDGFD and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGFD concentration in each sample is interpolated from this standard curve.
  • Human Platelet-derived growth factor D, receptor-binding form Protein information
  • Uniprot ID PDGFD_HUMAN
  • Uniprot AC Q9GZP0; A8K9T6; Q9BWV5;
  • UniGene Hs.352298;
  • GeneID 80310
  • KEGG hsa:80310;
  • Human Platelet-derived growth factor D, receptor-binding form Protein SEQUENCE

  • SEQUENCE 370 AA; 42848 MW; D387F485E7BB7674 CRC64;

    MHRLIFVYTL ICANFCSCRD TSATPQSASI KALRNANLRR DESNHLTDLY

    RRDETIQVKG NGYVQSPRFP NSYPRNLLLT WRLHSQENTR IQLVFDNQFG

    LEEAENDICR YDFVEVEDIS ETSTIIRGRW CGHKEVPPRI KSRTNQIKIT

    FKSDDYFVAK PGFKIYYSLL EDFQPAAASE TNWESVTSSI SGVSYNSPSV

    TDPTLIADAL DKKIAEFDTV EDLLKYFNPE SWQEDLENMY LDTPRYRGRS

    YHDRKSKVDL DRLNDDAKRY SCTPRNYSVN IREELKLANV VFFPRCLLVQ

    RCGGNCGCGT VNWRSCTCNS GKTVKKYHEV LQFEPGHIKR RGRAKTMALV

    DIQLDHHERC DCICSSRPPR

  • UCSC uc001php.4; human. [Q9GZP0-1];



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