Human PAFAH1B3 ELISA Kit

Alternative name
Human PAF acetylhydrolase 29 kDa subunit ELISA Kit;Human PAF-AH 29 kDa subunit ELISA Kit;Human PAF-AH subunit gamma ELISA Kit;Human PAFAH subunit gamma ELISA Kit;Human PAFAHG ELISA Kit;Human platelet activating factor acetylhydrolase 1b catalytic subunit 3 ELISA Kit;Human platelet-activating factor acetylhydrolase IB subunit gamma ELISA Kit;Human PAF-AH1b alpha 1 subunit ELISA Kit;Human platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa) ELISA Kit;Human platelet-activating factor acetylhydrolase, isoform Ib, subunit 3 (29kDa) ELISA Kit;
Catalog
E029636
species
Human
GenePAFAH1B3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PAFAH1B3 ELISA Kit allows for the in vitro quantitative determination of PAFAH1B3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: PAFAH1B3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAFAH1B3 antibody and an PAFAH1B3-HRP conjugate. The assay sample and buffer are incubated together with PAFAH1B3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAFAH1B3 concentration since PAFAH1B3 from samples and PAFAH1B3-HRP conjugate compete for the anti-PAFAH1B3 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAFAH1B3 from the sample, fewer sites are left to bind PAFAH1B3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAFAH1B3 concentration in each sample is interpolated from this standard curve.
Human Platelet-activating factor acetylhydrolase IB subunit gamma Protein information
Uniprot ID PA1B3_HUMAN
Uniprot AC Q15102; Q53X88;
UniGene Hs.466831;
GeneID 5050
KEGG hsa:5050;
Human Platelet-activating factor acetylhydrolase IB subunit gamma Protein SEQUENCE
SEQUENCE 231 AA; 25734 MW; 58A4CB8E7076AE23 CRC64;
MSGEENPASK PTPVQDVQGD GRWMSLHHRF VADSKDKEPE VVFIGDSLVQ
LMHQCEIWRE LFSPLHALNF GIGGDGTQHV LWRLENGELE HIRPKIVVVW
VGTNNHGHTA EQVTGGIKAI VQLVNERQPQ ARVVVLGLLP RGQHPNPLRE
KNRQVNELVR AALAGHPRAH FLDADPGFVH SDGTISHHDM YDYLHLSRLG
YTPVCRALHS LLLRLLAQDQ GQGAPLLEPA P
UCSC uc002otg.3; human.;

Category

Human PAFAH1B3 ELISA Kit