Human PAFAH1B3 ELISA Kit

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  • Alternative name

    Human PAF acetylhydrolase 29 kDa subunit ELISA Kit;Human PAF-AH 29 kDa subunit ELISA Kit;Human PAF-AH subunit gamma ELISA Kit;Human PAFAH subunit gamma ELISA Kit;Human PAFAHG ELISA Kit;Human platelet activating factor acetylhydrolase 1b catalytic subunit 3 ELISA Kit;Human platelet-activating factor acetylhydrolase IB subunit gamma ELISA Kit;Human PAF-AH1b alpha 1 subunit ELISA Kit;Human platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa) ELISA Kit;Human platelet-activating factor acetylhydrolase, isoform Ib, subunit 3 (29kDa) ELISA Kit;

  • Catalog
    E029636
  • species
    Human
  • GenePAFAH1B3
  • Standard CurveHuman PAFAH1B3 ELISA Kit
  • Other Species Mouse Pafah1b3 ELISA KitBovine PAFAH1B3 ELISA KitRat Pafah1b3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PAFAH1B3 ELISA Kit allows for the in vitro quantitative determination of PAFAH1B3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: PAFAH1B3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAFAH1B3 antibody and an PAFAH1B3-HRP conjugate. The assay sample and buffer are incubated together with PAFAH1B3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAFAH1B3 concentration since PAFAH1B3 from samples and PAFAH1B3-HRP conjugate compete for the anti-PAFAH1B3 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAFAH1B3 from the sample, fewer sites are left to bind PAFAH1B3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAFAH1B3 concentration in each sample is interpolated from this standard curve.
  • Human Platelet-activating factor acetylhydrolase IB subunit gamma Protein information
  • Uniprot ID PA1B3_HUMAN
  • Uniprot AC Q15102; Q53X88;
  • UniGene Hs.466831;
  • GeneID 5050
  • KEGG hsa:5050;
  • Human Platelet-activating factor acetylhydrolase IB subunit gamma Protein SEQUENCE
  • SEQUENCE 231 AA; 25734 MW; 58A4CB8E7076AE23 CRC64;

    MSGEENPASK PTPVQDVQGD GRWMSLHHRF VADSKDKEPE VVFIGDSLVQ

    LMHQCEIWRE LFSPLHALNF GIGGDGTQHV LWRLENGELE HIRPKIVVVW

    VGTNNHGHTA EQVTGGIKAI VQLVNERQPQ ARVVVLGLLP RGQHPNPLRE

    KNRQVNELVR AALAGHPRAH FLDADPGFVH SDGTISHHDM YDYLHLSRLG

    YTPVCRALHS LLLRLLAQDQ GQGAPLLEPA P

  • UCSC uc002otg.3; human.;


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