Human PLS1 ELISA Kit

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  • Alternative name

    Human Intestine-specific plastin ELISA Kit;Human I-plastin ELISA Kit;Human plastin 1 ELISA Kit;Human plastin-1 ELISA Kit;Human I plastin ELISA Kit;Human fimbrin ELISA Kit;Human intestine specific plastin ELISA Kit;

  • Catalog
    E029622
  • species
    Human
  • GenePLS1
  • Standard CurveHuman PLS1 ELISA Kit
  • Other Species Mouse Pls1 ELISA KitBovine PLS1 ELISA KitChicken PLS1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Plastin 1 (PLS1). No significant cross-reactivity or interference between Plastin 1 (PLS1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.115ng/mL.
  • Intended UseHuman PLS1 ELISA Kit allows for the in vitro quantitative determination of PLS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plastin 1 (PLS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plastin 1 (PLS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plastin 1 (PLS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plastin 1 (PLS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Plastin-1 Protein information
  • Uniprot ID PLSI_HUMAN
  • Uniprot AC Q14651; A8K2Q1; D3DNG3; Q8NEG6;
  • UniGene Hs.203637;
  • GeneID 5357
  • KEGG hsa:5357;
  • Human Plastin-1 Protein SEQUENCE
  • SEQUENCE 629 AA; 70253 MW; F904031933C2C323 CRC64;

    MENSTTTISR EELEELQEAF NKIDIDNSGY VSDYELQDLF KEASLPLPGY

    KVREIVEKIL SVADSNKDGK ISFEEFVSLM QELKSKDISK TFRKIINKRE

    GITAIGGTST ISSEGTQHSY SEEEKVAFVN WINKALENDP DCKHLIPMNP

    NDDSLFKSLA DGILLCKMIN LSEPDTIDER AINKKKLTPF TISENLNLAL

    NSASAIGCTV VNIGASDLKE GKPHLVLGLL WQIIKVGLFA DIEISRNEAL

    IALLNEGEEL EELMKLSPEE LLLRWVNYHL TNAGWHTISN FSQDIKDSRA

    YFHLLNQIAP KGGEDGPAIA IDLSGINETN DLKRAGLMLQ EADKLGCKQF

    VTPADVVSGN PKLNLAFVAN LFNTYPCLHK PNNNDIDMNL LEGESKEERT

    FRNWMNSLGV NPYINHLYSD LADALVIFQL YEMIRVPVNW SHVNKPPYPA

    LGGNMKKIEN CNYAVELGKN KAKFSLVGIA GQDLNEGNST LTLALVWQLM

    RRYTLNVLSD LGEGEKVNDE IIIKWVNQTL KSANKKTSIS SFKDKSISTS

    LPVLDLIDAI APNAVRQEMI RRENLSDEDK LNNAKYAISV ARKIGARIYA

    LPDDLVEVKP KMVMTVFACL MGKGLNRIK

  • UCSC uc003euz.4; human.;


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