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Human KLKB1 ELISA Kit

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  • Alternative name

    Human Fletcher factor ELISA Kit;Human kininogenin ELISA Kit;Human plasma prekallikrein ELISA Kit;Human PKK ELISA Kit;Human KLK3 ELISA Kit;Human PKKD ELISA Kit;Human PPK ELISA Kit;Human kallikrein B1 ELISA Kit;Human plasma kallikrein ELISA Kit;Human kallikrein B, plasma (Fletcher factor) 1 ELISA Kit;
  • Catalog
    E029565
  • species
    Human
  • GeneKLKB1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of KLKB1. No significant cross-reactivity or interference between KLKB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KLKB1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman KLKB1 ELISA Kit allows for the in vitro quantitative determination of KLKB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical    Principle of the assay: KLKB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLKB1 antibody and an KLKB1-HRP conjugate. The assay sample and buffer are incubated together with KLKB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLKB1 concentration since KLKB1 from samples and KLKB1-HRP conjugate compete for the anti-KLKB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLKB1 from the sample, fewer sites are left to bind KLKB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLKB1 concentration in each sample is interpolated from this standard curve.
  • Human Plasma kallikrein light chain Protein information
  • Uniprot ID KLKB1_HUMAN
  • Uniprot AC P03952; A6NH96; B2R8H9; Q17RE8; Q17RE9; Q4W5C3;
  • UniGene Hs.237642;
  • GeneID 3818
  • KEGG hsa:3818;
  • Human Plasma kallikrein light chain Protein SEQUENCE

  • SEQUENCE 638 AA; 71370 MW; E62F9C1053838FB4 CRC64;

    MILFKQATYF ISLFATVSCG CLTQLYENAF FRGGDVASMY TPNAQYCQMR

    CTFHPRCLLF SFLPASSIND MEKRFGCFLK DSVTGTLPKV HRTGAVSGHS

    LKQCGHQISA CHRDIYKGVD MRGVNFNVSK VSSVEECQKR CTNNIRCQFF

    SYATQTFHKA EYRNNCLLKY SPGGTPTAIK VLSNVESGFS LKPCALSEIG

    CHMNIFQHLA FSDVDVARVL TPDAFVCRTI CTYHPNCLFF TFYTNVWKIE

    SQRNVCLLKT SESGTPSSST PQENTISGYS LLTCKRTLPE PCHSKIYPGV

    DFGGEELNVT FVKGVNVCQE TCTKMIRCQF FTYSLLPEDC KEEKCKCFLR

    LSMDGSPTRI AYGTQGSSGY SLRLCNTGDN SVCTTKTSTR IVGGTNSSWG

    EWPWQVSLQV KLTAQRHLCG GSLIGHQWVL TAAHCFDGLP LQDVWRIYSG

    ILNLSDITKD TPFSQIKEII IHQNYKVSEG NHDIALIKLQ APLNYTEFQK

    PICLPSKGDT STIYTNCWVT GWGFSKEKGE IQNILQKVNI PLVTNEECQK

    RYQDYKITQR MVCAGYKEGG KDACKGDSGG PLVCKHNGMW RLVGITSWGE

    GCARREQPGV YTKVAEYMDW ILEKTQSSDG KAQMQSPA

  • UCSC uc003iyy.4; human.;



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