Human PKP2 ELISA Kit

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  • Alternative name

    Human ARVD9 ELISA Kit;Human plakophilin 2 ELISA Kit;Human plakophilin-2 ELISA Kit;

  • Catalog
    E029551
  • species
    Human
  • GenePKP2
  • Standard CurveHuman PKP2 ELISA Kit
  • Other Species Mouse PKP2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PKP2. No significant cross-reactivity or interference between PKP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PKP2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PKP2 ELISA Kit allows for the in vitro quantitative determination of PKP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: PKP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKP2 antibody and an PKP2-HRP conjugate. The assay sample and buffer are incubated together with PKP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKP2 concentration since PKP2 from samples and PKP2-HRP conjugate compete for the anti-PKP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PKP2 from the sample, fewer sites are left to bind PKP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKP2 concentration in each sample is interpolated from this standard curve.
  • Human Plakophilin-2 Protein information
  • Uniprot ID PKP2_HUMAN
  • Uniprot AC Q99959; A0AV37; B8QFA1; B8QGS6; B8QGS7; D3DUW9; Q4VC01; Q99960;
  • UniGene Hs.164384;
  • GeneID 5318
  • KEGG hsa:5318;
  • Human Plakophilin-2 Protein SEQUENCE
  • SEQUENCE 881 AA; 97415 MW; 947838B0C8275D5D CRC64;

    MAAPGAPAEY GYIRTVLGQQ ILGQLDSSSL ALPSEAKLKL AGSSGRGGQT

    VKSLRIQEQV QQTLARKGRS SVGNGNLHRT SSVPEYVYNL HLVENDFVGG

    RSPVPKTYDM LKAGTTATYE GRWGRGTAQY SSQKSVEERS LRHPLRRLEI

    SPDSSPERAH YTHSDYQYSQ RSQAGHTLHH QESRRAALLV PPRYARSEIV

    GVSRAGTTSR QRHFDTYHRQ YQHGSVSDTV FDSIPANPAL LTYPRPGTSR

    SMGNLLEKEN YLTAGLTVGQ VRPLVPLQPV TQNRASRSSW HQSSFHSTRT

    LREAGPSVAV DSSGRRAHLT VGQAAAGGSG NLLTERSTFT DSQLGNADME

    MTLERAVSML EADHMLPSRI SAAATFIQHE CFQKSEARKR VNQLRGILKL

    LQLLKVQNED VQRAVCGALR NLVFEDNDNK LEVAELNGVP RLLQVLKQTR

    DLETKKQITD HTVNLRSRNG WPGAVAHACN PSTLGGQGGR ITRSGVRDQP

    DQHGLLWNLS SNDKLKNLMI TEALLTLTEN IIIPFSGWPE GDYPKANGLL

    DFDIFYNVTG CLRNMSSAGA DGRKAMRRCD GLIDSLVHYV RGTIADYQPD

    DKATENCVCI LHNLSYQLEA ELPEKYSQNI YIQNRNIQTD NNKSIGCFGS

    RSRKVKEQYQ DVPMPEEKSN PKGVEWLWHS IVIRMYLSLI AKSVRNYTQE

    ASLGALQNLT AGSGPMPTSV AQTVVQKESG LQHTRKMLHV GDPSVKKTAI

    SLLRNLSRNL SLQNEIAKET LPDLVSIIPD TVPSTDLLIE TTASACYTLN

    NIIQNSYQNA RDLLNTGGIQ KIMAISAGDA YASNKASKAA SVLLYSLWAH

    TELHHAYKKA QFKKTDFVNS RTAKAYHSLK D

  • UCSC uc001rlj.5; human. [Q99959-1];



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