Human PKP1 ELISA Kit

Alternative name
Human band 6 protein ELISA Kit;Human B6P ELISA Kit;Human plakophilin 1 ELISA Kit;Human plakophilin-1 ELISA Kit;
Catalog
E029550
species
Human
GenePKP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Plakophilin 1 (PKP1). No significant cross-reactivity or interference between Plakophilin 1 (PKP1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.125ng/mL.
Intended UseHuman PKP1 ELISA Kit allows for the in vitro quantitative determination of PKP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plakophilin 1 (PKP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plakophilin 1 (PKP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plakophilin 1 (PKP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plakophilin 1 (PKP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Plakophilin-1 Protein information
Uniprot ID PKP1_HUMAN
Uniprot AC YTVRNLMA SQPQLAKQYF SSSMLNNIIN LCRSSASPKA AEAARLLLSD MWSSKELQGV
UniGene Hs.497350;
GeneID 5317
KEGG hsa:5317;
Human Plakophilin-1 Protein SEQUENCE
SEQUENCE 747 AA; 82861 MW; 60C1BCCC50AB4E6F CRC64;
MNHSPLKTAL AYECFQDQDN STLALPSDQK MKTGTSGRQR VQEQVMMTVK
RQKSKSSQSS TLSHSNRGSM YDGLADNYNY GTTSRSSYYS KFQAGNGSWG
YPIYNGTLKR EPDNRRFSSY SQMENWSRHY PRGSCNTTGA GSDICFMQKI
KASRSEPDLY CDPRGTLRKG TLGSKGQKTT QNRYSFYSTC SGQKAIKKCP
VRPPSCASKQ DPVYIPPISC NKDLSFGHSR ASSKICSEDI ECSGLTIPKA
VQYLSSQDEK YQAIGAYYIQ HTCFQDESAK QQVYQLGGIC KLVDLLRSPN
QNVQQAAAGA LRNLVFRSTT NKLETRRQNG IREAVSLLRR TGNAEIQKQL
TGLLWNLSST DELKEELIAD ALPVLADRVI IPFSGWCDGN SNMSREVVDP
EVFFNATGCL RKRLGMRELL ALVPQRATSS RVNLSSADAG RQTMRNYSGL
IDSLMAYVQN CVAASRCDDK SVENCMCVLH NLSYRLDAEV PTRYRQLEYN
ARNAYTEKSS TGCFSNKSDK MMNNNYDCPL PEEETNPKGS GWLYHSDAIR
TYLNLMGKSK KDATLEACAG ALQNLTASKG LMSSGMSQLI GLKEKGLPQI
ARLLQSGNSD VVRSGASLLS NMSRHPLLHR VMGNQVFPEV TRLLTSHTGN
TSNSEDILSS ACYTVRNLMA SQPQLAKQYF SSSMLNNIIN LCRSSASPKA
AEAARLLLSD MWSSKELQGV LRQQGFDRNM LGTLAGANSL RNFTSRF
UCSC uc001gwd.3; human. [Q13835-1];

Category

Human PKP1 ELISA Kit

Human band 6 protein ELISA Kit;Human B6P ELISA Kit;Human plakophilin 1 ELISA Kit;Human plakophilin-1 ELISA Kit;