SpecificityThis assay has high sensitivity and excellent specificity for detection of Placenta Specific Protein 9 (PLAC9).
No significant cross-reactivity or interference between Placenta Specific Protein 9 (PLAC9) and analogues was observed.
Intended UseHuman PLAC9 ELISA Kit allows for the in vitro quantitative determination of PLAC9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Specific Protein 9 (PLAC9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placenta Specific Protein 9 (PLAC9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Specific Protein 9 (PLAC9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Specific Protein 9 (PLAC9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Placenta-specific protein 9 Protein information
Human Placenta-specific protein 9 Protein SEQUENCE