Intended UseHuman ANNA1 ELISA Kit allows for the in vitro quantitative determination of ANNA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. DNA was precoated onto 96-well plates. And the Biotin- labeled DNA was used as detection antigen. The standards, test samples and Biotin- labeled DNA were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANNA1 amount of sample captured in plate. Read the OD absorbance at 450nm in a microplate reader, and then the concentration of ANNA1 can be calculated.