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Human PIM3 ELISA Kit

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  • Alternative name

    Human pim-3 ELISA Kit;Human Pim-3 proto-oncogene, serine/threonine kinase ELISA Kit;Human serine/threonine-protein kinase pim-3 ELISA Kit;Human pim-3 oncogene ELISA Kit;Human serine/threonine kinase Pim-3 ELISA Kit;
  • Catalog
    E029419
  • species
    Human
  • GenePIM3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Pim-3 Oncogene (PIM3). No significant cross-reactivity or interference between Pim-3 Oncogene (PIM3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.228ng/mL.
  • Intended UseHuman PIM3 ELISA Kit allows for the in vitro quantitative determination of PIM3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-3 Oncogene (PIM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-3 Oncogene (PIM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pim-3 Oncogene (PIM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pim-3 Oncogene (PIM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serine/threonine-protein kinase pim-3 Protein information
  • Uniprot ID PIM3_HUMAN
  • Uniprot AC Q86V86; A5D8X8; A8K7J0; B1B0P0; Q68BM2;
  • UniGene Hs.530381;
  • GeneID 415116
  • KEGG hsa:415116;
  • Human Serine/threonine-protein kinase pim-3 Protein SEQUENCE

  • SEQUENCE 326 AA; 35891 MW; 41FDEFAC4367A162 CRC64;

    MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKAY QVGAVLGSGG

    FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGATVPLE VVLLRKVGAA

    GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF

    FAQVLAAVRH CHSCGVVHRD IKDENLLVDL RSGELKLIDF GSGALLKDTV

    YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE

    ILRGRLLFRR RVSPECQQLI RWCLSLRPSE RPSLDQIAAH PWMLGADGGV

    PESCDLRLCT LDPDDVASTT SSSESL

  • UCSC uc003bjb.4; human.;



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