Human ACP2 ELISA Kit

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  • Alternative name

    Human LAP ELISA Kit;Human acid phosphatase 2, lysosomal ELISA Kit;Human lysosomal acid phosphatase ELISA Kit;

  • Catalog
    E000294
  • species
    Human
  • GeneACP2
  • Standard CurveHuman ACP2 ELISA Kit
  • Other Species Mouse Acp2 ELISA KitBovine ACP2 ELISA KitMonkey ACP2 ELISA KitRat Acp2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Acid Phosphatase 2, Lysosomal (ACP2). No significant cross-reactivity or interference between Acid Phosphatase 2, Lysosomal (ACP2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.55ng/mL.
  • Intended UseHuman ACP2 ELISA Kit allows for the in vitro quantitative determination of ACP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 2, Lysosomal (ACP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 2, Lysosomal (ACP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 2, Lysosomal (ACP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acid Phosphatase 2, Lysosomal (ACP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Lysosomal acid phosphatase Protein information
  • Uniprot ID PPAL_HUMAN
  • Uniprot AC P11117; E9PCI1; Q561W5; Q9BTU7;
  • UniGene Hs.532492;
  • GeneID 53
  • KEGG hsa:53;
  • Human Lysosomal acid phosphatase Protein SEQUENCE
  • SEQUENCE 423 AA; 48344 MW; 3431A30B83A1E2B4 CRC64;

    MAGKRSGWSR AALLQLLLGV NLVVMPPTRA RSLRFVTLLY RHGDRSPVKT

    YPKDPYQEEE WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV

    YVRSTDFDRT LMSAEANLAG LFPPNGMQRF NPNISWQPIP VHTVPITEDR

    LLKFPLGPCP RYEQLQNETR QTPEYQNESS RNAQFLDMVA NETGLTDLTL

    ETVWNVYDTL FCEQTHGLRL PPWASPQTMQ RLSRLKDFSF RFLFGIYQQA

    EKARLQGGVL LAQIRKNLTL MATTSQLPKL LVYSAHDTTL VALQMALDVY

    NGEQAPYASC HIFELYQEDS GNFSVEMYFR NESDKAPWPL SLPGCPHRCP

    LQDFLRLTEP VVPKDWQQEC QLASGPADTE VIVALAVCGS ILFLLIVLLL

    TVLFRMQAQP PGYRHVADGE DHA

  • UCSC uc001nei.3; human. [P11117-1];


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