Human ACP2 ELISA Kit

Alternative name
Human LAP ELISA Kit;Human acid phosphatase 2, lysosomal ELISA Kit;Human lysosomal acid phosphatase ELISA Kit;
Catalog
E000294
species
Human
GeneACP2
Standard Curve
Other Species Mouse Acp2 ELISA Kit Bovine ACP2 ELISA Kit Monkey ACP2 ELISA Kit Rat Acp2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Acid Phosphatase 2, Lysosomal (ACP2). No significant cross-reactivity or interference between Acid Phosphatase 2, Lysosomal (ACP2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.55ng/mL.
Intended UseHuman ACP2 ELISA Kit allows for the in vitro quantitative determination of ACP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 2, Lysosomal (ACP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 2, Lysosomal (ACP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 2, Lysosomal (ACP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acid Phosphatase 2, Lysosomal (ACP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Lysosomal acid phosphatase Protein information
Uniprot ID PPAL_HUMAN
Uniprot AC P11117; E9PCI1; Q561W5; Q9BTU7;
UniGene Hs.532492;
GeneID 53
KEGG hsa:53;
Human Lysosomal acid phosphatase Protein SEQUENCE
SEQUENCE 423 AA; 48344 MW; 3431A30B83A1E2B4 CRC64;
MAGKRSGWSR AALLQLLLGV NLVVMPPTRA RSLRFVTLLY RHGDRSPVKT
YPKDPYQEEE WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV
YVRSTDFDRT LMSAEANLAG LFPPNGMQRF NPNISWQPIP VHTVPITEDR
LLKFPLGPCP RYEQLQNETR QTPEYQNESS RNAQFLDMVA NETGLTDLTL
ETVWNVYDTL FCEQTHGLRL PPWASPQTMQ RLSRLKDFSF RFLFGIYQQA
EKARLQGGVL LAQIRKNLTL MATTSQLPKL LVYSAHDTTL VALQMALDVY
NGEQAPYASC HIFELYQEDS GNFSVEMYFR NESDKAPWPL SLPGCPHRCP
LQDFLRLTEP VVPKDWQQEC QLASGPADTE VIVALAVCGS ILFLLIVLLL
TVLFRMQAQP PGYRHVADGE DHA
UCSC uc001nei.3; human. [P11117-1];

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Category

Human ACP2 ELISA Kit

Human LAP ELISA Kit;Human acid phosphatase 2, lysosomal ELISA Kit;Human lysosomal acid phosphatase ELISA Kit;