Human PMVK ELISA Kit

Alternative name
Human PMKASE ELISA Kit;Human hPMK ELISA Kit;Human PMKI ELISA Kit;Human HUMPMKI ELISA Kit;Human PMK ELISA Kit;Human PMKA ELISA Kit;Human POROK1 ELISA Kit;Human phosphomevalonate kinase ELISA Kit;Human testis tissue sperm-binding protein Li 95mP ELISA Kit;
Catalog
E029278
species
Human
GenePMVK
SpecificityThis assay has high sensitivity and excellent specificity for detection of PMVK. No significant cross-reactivity or interference between PMVK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PMVK and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PMVK ELISA Kit allows for the in vitro quantitative determination of PMVK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: PMVK ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-PMVK antibody and an PMVK-HRP conjugate. The assay sample and buffer are incubated together with PMVK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PMVK concentration since PMVK from samples and PMVK-HRP conjugate compete for the anti-PMVK antibody binding site. Since the number of sites is limited, as more sites are occupied by PMVK from the sample, fewer sites are left to bind PMVK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PMVK concentration in each sample is interpolated from this standard curve.
Human Phosphomevalonate kinase Protein information
Uniprot ID PMVK_HUMAN
Uniprot AC Q15126; Q5TZW9;
UniGene Hs.30954;
GeneID 10654
KEGG hsa:10654;
Human Phosphomevalonate kinase Protein SEQUENCE
SEQUENCE 192 AA; 21995 MW; D7E720D0DDCCA249 CRC64;
MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ
YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS
QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD
DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL

Category

Human PMVK ELISA Kit

Human PMKASE ELISA Kit;Human hPMK ELISA Kit;Human PMKI ELISA Kit;Human HUMPMKI ELISA Kit;Human PMK ELISA Kit;Human PMKA ELISA Kit;Human POROK1 ELISA Kit;Human phosphomevalonate kinase ELISA Kit;Human testis tissue sperm-binding protein Li 95mP ELISA Kit;