Human PMVK ELISA Kit

Contact Us
  • Alternative name

    Human PMKASE ELISA Kit;Human hPMK ELISA Kit;Human PMKI ELISA Kit;Human HUMPMKI ELISA Kit;Human PMK ELISA Kit;Human PMKA ELISA Kit;Human POROK1 ELISA Kit;Human phosphomevalonate kinase ELISA Kit;Human testis tissue sperm-binding protein Li 95mP ELISA Kit;

  • Catalog
    E029278
  • species
    Human
  • GenePMVK
  • Standard CurveHuman PMVK ELISA Kit
  • Other Species Mouse Pmvk ELISA KitBovine PMVK ELISA KitPorcine PMVK ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PMVK. No significant cross-reactivity or interference between PMVK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PMVK and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PMVK ELISA Kit allows for the in vitro quantitative determination of PMVK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: PMVK ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-PMVK antibody and an PMVK-HRP conjugate. The assay sample and buffer are incubated together with PMVK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PMVK concentration since PMVK from samples and PMVK-HRP conjugate compete for the anti-PMVK antibody binding site. Since the number of sites is limited, as more sites are occupied by PMVK from the sample, fewer sites are left to bind PMVK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PMVK concentration in each sample is interpolated from this standard curve.
  • Human Phosphomevalonate kinase Protein information
  • Uniprot ID PMVK_HUMAN
  • Uniprot AC Q15126; Q5TZW9;
  • UniGene Hs.30954;
  • GeneID 10654
  • KEGG hsa:10654;
  • Human Phosphomevalonate kinase Protein SEQUENCE
  • SEQUENCE 192 AA; 21995 MW; D7E720D0DDCCA249 CRC64;

    MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ

    YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS

    QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD

    DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL



Related products with Human PMVK ELISA Kit

Inquiry About Human PMVK ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!