Human PLSCR2 ELISA Kit

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  • Alternative name

    Human PL scramblase 2 ELISA Kit;Human ca(2+)-dependent phospholipid scramblase 2 ELISA Kit;Human phospholipid scramblase 2 ELISA Kit;

  • Catalog
    E029258
  • species
    Human
  • GenePLSCR2
  • Other Species Mouse PLSCR2 ELISA KitBovine PLSCR2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PLSCR2 ELISA Kit allows for the in vitro quantitative determination of PLSCR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: PLSCR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLSCR2 antibody and an PLSCR2-HRP conjugate. The assay sample and buffer are incubated together with PLSCR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLSCR2 concentration since PLSCR2 from samples and PLSCR2-HRP conjugate compete for the anti-PLSCR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLSCR2 from the sample, fewer sites are left to bind PLSCR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLSCR2 concentration in each sample is interpolated from this standard curve.
  • Human Phospholipid scramblase 2 Protein information
  • Uniprot ID PLS2_HUMAN
  • Uniprot AC Q9NRY7; B4DXC3; J3KR76; Q0VAQ1; Q6NSW9; Q7Z4L7;
  • UniGene Hs.744414;
  • GeneID 57047
  • KEGG hsa:57047;
  • Human Phospholipid scramblase 2 Protein SEQUENCE
  • SEQUENCE 297 AA; 33504 MW; 58F50C24AB438729 CRC64;

    MRSWNSLFCL NSSRPPGHIV YPKHQAGHTG KQADHLGSQA FYPGRQHDYL

    VPPAGTAGIP VQNQPGRPEG VPWMPAPPPP LNCPPGLEYL SQIDMILIHQ

    QIELLEVLFS FESSNMYEIK NSFGQRIYFA AEDTNFCIRN CCGRSRPFTL

    RITDNVGREV ITLERPLRCN CCCCPCCLQE IEIQAPPGVP VGYVTQTWHP

    CLTKFTIKNQ KREDVLKISG PCIVCSCIAG VDFEITSLDE QIVVGRISKH

    WSGFLREAFT DADNFGIQFP RDLDVKMKAV MIGACFLIDY MFFERTR

  • UCSC uc003evv.3; human. [Q9NRY7-2];


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