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Human PLCG1 ELISA Kit

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  • Alternative name

    Human PLC-148 ELISA Kit;Human phosphoinositide phospholipase C-gamma-1 ELISA Kit;Human phospholipase C-II ELISA Kit;Human PLC-II ELISA Kit;Human Phospholipase C-gamma-1 ELISA Kit;Human PLC-gamma-1 ELISA Kit;Human PLC1 ELISA Kit;Human NCKAP3 ELISA Kit;Human PLC148 ELISA Kit;Human PLCgamma1 ELISA Kit;Human phospholipase C gamma 1 ELISA Kit;Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 ELISA Kit;Human 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate ELISA Kit;Human inositoltrisphosphohydrolase ELISA Kit;Human monophosphatidylinositol phosphodiesterase ELISA Kit;Human phosphatidylinositol phospholipase C ELISA Kit;Human phosphoinositidase C ELISA Kit;Human phosphoinositide phospholipase C ELISA Kit;Human phospholipase C, gamma 1 (formerly subtype 148) ELISA Kit;Human phospholipase C-148 ELISA Kit;Human triphosphoinositide phosphodiesterase ELISA Kit;
  • Catalog
    E029207
  • species
    Human
  • GenePLCG1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PLCG1 ELISA Kit allows for the in vitro quantitative determination of PLCG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical    Principle of the Assay: PLCg1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLCg1. Standards or samples are then added to the microtiter plate wells and PLCg1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLCg1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PLCg1 are added to each well to "sandwich" the PLCg1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLCg1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLCg1 concentration in each sample is interpolated from this standard curve.
  • Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Protein information
  • Uniprot ID PLCG1_HUMAN
  • Uniprot AC P19174; B7ZLY7; B9EGH4; E1P5W4; Q2V575;
  • UniGene Hs.268177;
  • GeneID 5335
  • KEGG hsa:5335;
  • Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Protein SEQUENCE

  • SEQUENCE 1290 AA; 148532 MW; AE05ABE2A18EDDAC CRC64;

    MAGAASPCAN GCGPGAPSDA EVLHLCRSLE VGTVMTLFYS KKSQRPERKT

    FQVKLETRQI TWSRGADKIE GAIDIREIKE IRPGKTSRDF DRYQEDPAFR

    PDQSHCFVIL YGMEFRLKTL SLQATSEDEV NMWIKGLTWL MEDTLQAPTP

    LQIERWLRKQ FYSVDRNRED RISAKDLKNM LSQVNYRVPN MRFLRERLTD

    LEQRSGDITY GQFAQLYRSL MYSAQKTMDL PFLEASTLRA GERPELCRVS

    LPEFQQFLLD YQGELWAVDR LQVQEFMLSF LRDPLREIEE PYFFLDEFVT

    FLFSKENSVW NSQLDAVCPD TMNNPLSHYW ISSSHNTYLT GDQFSSESSL

    EAYARCLRMG CRCIELDCWD GPDGMPVIYH GHTLTTKIKF SDVLHTIKEH

    AFVASEYPVI LSIEDHCSIA QQRNMAQYFK KVLGDTLLTK PVEISADGLP

    SPNQLKRKIL IKHKKLAEGS AYEEVPTSMM YSENDISNSI KNGILYLEDP

    VNHEWYPHYF VLTSSKIYYS EETSSDQGNE DEEEPKEVSS STELHSNEKW

    FHGKLGAGRD GRHIAERLLT EYCIETGAPD GSFLVRESET FVGDYTLSFW

    RNGKVQHCRI HSRQDAGTPK FFLTDNLVFD SLYDLITHYQ QVPLRCNEFE

    MRLSEPVPQT NAHESKEWYH ASLTRAQAEH MLMRVPRDGA FLVRKRNEPN

    SYAISFRAEG KIKHCRVQQE GQTVMLGNSE FDSLVDLISY YEKHPLYRKM

    KLRYPINEEA LEKIGTAEPD YGALYEGRNP GFYVEANPMP TFKCAVKALF

    DYKAQREDEL TFIKSAIIQN VEKQEGGWWR GDYGGKKQLW FPSNYVEEMV

    NPVALEPERE HLDENSPLGD LLRGVLDVPA CQIAIRPEGK NNRLFVFSIS

    MASVAHWSLD VAADSQEELQ DWVKKIREVA QTADARLTEG KIMERRKKIA

    LELSELVVYC RPVPFDEEKI GTERACYRDM SSFPETKAEK YVNKAKGKKF

    LQYNRLQLSR IYPKGQRLDS SNYDPLPMWI CGSQLVALNF QTPDKPMQMN

    QALFMTGRHC GYVLQPSTMR DEAFDPFDKS SLRGLEPCAI SIEVLGARHL

    PKNGRGIVCP FVEIEVAGAE YDSTKQKTEF VVDNGLNPVW PAKPFHFQIS

    NPEFAFLRFV VYEEDMFSDQ NFLAQATFPV KGLKTGYRAV PLKNNYSEDL

    ELASLLIKID IFPAKENGDL SPFSGTSLRE RGSDASGQLF HGRAREGSFE

    SRYQQPFEDF RISQEHLADH FDSRERRAPR RTRVNGDNRL

  • UCSC uc002xjo.2; human. [P19174-1];



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