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Human PLA2G12B ELISA Kit

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  • Alternative name

    Human Group XIII secretory phospholipase A2-like protein ELISA Kit;Human GXIII sPLA2-like ELISA Kit;Human sPLA2-GXIIB ELISA Kit;Human GXIIB ELISA Kit;Human PLA2G13 ELISA Kit;Human FKSG71 ELISA Kit;Human GXIIIsPLA2 ELISA Kit;Human phospholipase A2 group XIIB ELISA Kit;Human group XIIB secretory phospholipase A2-like protein ELISA Kit;Human group XIII secreted phospholipase A2 ELISA Kit;Human phospholipase A2, group XIII ELISA Kit;
  • Catalog
    E029189
  • species
    Human
  • GenePLA2G12B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group XIIB (PLA2G12B). No significant cross-reactivity or interference between Phospholipase A2, Group XIIB (PLA2G12B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman PLA2G12B ELISA Kit allows for the in vitro quantitative determination of PLA2G12B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group XIIB (PLA2G12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group XIIB (PLA2G12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Group XIIB secretory phospholipase A2-like protein Protein information
  • Uniprot ID PG12B_HUMAN
  • Uniprot AC Q9BX93; B7ZL23; Q52LB2; Q96Q99;
  • UniGene Hs.333175;
  • GeneID 84647
  • KEGG hsa:84647;
  • Human Group XIIB secretory phospholipase A2-like protein Protein SEQUENCE

  • SEQUENCE 195 AA; 21659 MW; C00C817F81D68A70 CRC64;

    MKLASGFLVL WLSLGGGLAQ SDTSPDTEES YSDWGLRHLR GSFESVNSYF

    DSFLELLGGK NGVCQYRCRY GKAPMPRPGY KPQEPNGCGS YFLGLKVPES

    MDLGIPAMTK CCNQLDVCYD TCGANKYRCD AKFRWCLHSI CSDLKRSLGF

    VSKVEAACDS LVDTVFNTVW TLGCRPFMNS QRAACICAEE EKEEL

  • UCSC uc001jtf.2; human. [Q9BX93-1];



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