Human PLA2G4D ELISA Kit

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  • Alternative name

    Human cPLA2-delta ELISA Kit;Human phospholipase A2 group IVD ELISA Kit;Human cPLA2delta ELISA Kit;Human cytosolic phospholipase A2 delta ELISA Kit;Human phospholipase A2 delta, cytosolic ELISA Kit;Human phospholipase A2, group IVD (cytosolic) ELISA Kit;

  • Catalog
    E029176
  • species
    Human
  • GenePLA2G4D
  • Standard CurveHuman PLA2G4D ELISA Kit
  • Other Species Mouse Pla2g4d ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group IVD (PLA2G4D). No significant cross-reactivity or interference between Phospholipase A2, Group IVD (PLA2G4D) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman PLA2G4D ELISA Kit allows for the in vitro quantitative determination of PLA2G4D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IVD (PLA2G4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group IVD (PLA2G4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytosolic phospholipase A2 delta Protein information
  • Uniprot ID PA24D_HUMAN
  • Uniprot AC Q86XP0; Q8N176;
  • UniGene Hs.380225;
  • GeneID 283748
  • KEGG hsa:283748;
  • Human Cytosolic phospholipase A2 delta Protein SEQUENCE
  • SEQUENCE 818 AA; 91952 MW; 06187324748A4C21 CRC64;

    MESLSPGGPP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQ

    LSTAPGMKFK TKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTE

    DDICFKVLYD ISEVLPGKLL RKTFSQSPQG EEELDVEFLM EETSDRPENL

    ITNKVIVARE LSCLDVHLDS TGSTAVVADQ DKLELELVLK GSYEDTQTSF

    LGTASAFRFH YMAALETELS GRLRSSRSNG WNGDNSAGYL TVPLRPLTIG

    KEVTMDVPAP NAPGVRLQLK AEGCPEELAV HLGFNLCAEE QAFLSRRKQV

    VAKALKQALQ LDRDLQEDEV PVVGIMATGG GARAMTSLYG HLLALQKLGL

    LDCVTYFSGI SGSTWTMAHL YGDPEWSQRD LEGPIRYARE HLAKSKLEVF

    SPERLASYRR ELELRAEQGH PTTFVDLWAL VLESMLHGQV MDQKLSGQRA

    ALERGQNPLP LYLSLNVKEN NLETLDFKEW VEFSPYEVGF LKYGAFVPPE

    LFGSEFFMGR LMRRIPEPRI CFLEAIWSNI FSLNLLDAWY DLTSSGESWK

    QHIKDKTRSL EKEPLTTSGT SSRLEASWLQ PGTALAQAFK GFLTGRPLHQ

    RSPNFLQGLQ LHQDYCSHKD FSTWADYQLD SMPSQLTPKE PRLCLVDAAY

    FINTSSPSMF RPGRRLDLIL SFDYSLSAPF EALQQTELYC RARGLPFPRV

    EPSPQDQHQP RECHLFSDPA CPEAPILLHF PLVNASFKDH SAPGVQRSPA

    ELQGGQVDLT GATCPYTLSN MTYKEEDFER LLRLSDYNVQ TSQGAILQAL

    RTALKHRTLE ARPPRAQT

  • UCSC uc001zox.3; human. [Q86XP0-1];


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