SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group IID (PLA2G2D).
No significant cross-reactivity or interference between Phospholipase A2, Group IID (PLA2G2D) and analogues was observed.
Intended UseHuman PLA2G2D ELISA Kit allows for the in vitro quantitative determination of PLA2G2D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IID (PLA2G2D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IID (PLA2G2D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IID (PLA2G2D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group IID (PLA2G2D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Group IID secretory phospholipase A2 Protein information
Uniprot ID
PA2GD_HUMAN
Uniprot AC
Q9UNK4; A8K2Z1; B1AEL9; Q9UK01;
UniGene
Hs.189507;
GeneID
26279
KEGG
hsa:26279;
Human Group IID secretory phospholipase A2 Protein SEQUENCE