Human PLA2G2D ELISA Kit

Alternative name
Human GIID sPLA2 ELISA Kit;Human sPLA2-IID ELISA Kit;Human PLA2IID ELISA Kit;Human phosphatidylcholine 2-acylhydrolase 2D ELISA Kit;Human secretory-type PLA, stroma-associated homolog ELISA Kit;Human SPLASH ELISA Kit;Human sPLA2S ELISA Kit;Human phospholipase A2 group IID ELISA Kit;Human group IID secretory phospholipase A2 ELISA Kit;Human secretory phospholipase A2s ELISA Kit;
Catalog
E029169
species
Human
GenePLA2G2D
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group IID (PLA2G2D). No significant cross-reactivity or interference between Phospholipase A2, Group IID (PLA2G2D) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.32ng/mL.
Intended UseHuman PLA2G2D ELISA Kit allows for the in vitro quantitative determination of PLA2G2D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IID (PLA2G2D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IID (PLA2G2D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IID (PLA2G2D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group IID (PLA2G2D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Group IID secretory phospholipase A2 Protein information
Uniprot ID PA2GD_HUMAN
Uniprot AC Q9UNK4; A8K2Z1; B1AEL9; Q9UK01;
UniGene Hs.189507;
GeneID 26279
KEGG hsa:26279;
Human Group IID secretory phospholipase A2 Protein SEQUENCE
SEQUENCE 145 AA; 16546 MW; CF3A49DE516BD1EF CRC64;
MELALLCGLV VMAGVIPIQG GILNLNKMVK QVTGKMPILS YWPYGCHCGL
GGRGQPKDAT DWCCQTHDCC YDHLKTQGCS IYKDYYRYNF SQGNIHCSDK
GSWCEQQLCA CDKEVAFCLK RNLDTYQKRL RFYWRPHCRG QTPGC
UCSC uc001bcz.5; human.;

Human PLA2G2D ELISA Kit

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