Intended UseHuman MBL ELISA Kit allows for the in vitro quantitative determination of MBL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-MBL in human serum, plasma and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-MBL will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-MBL in the samples is then determined by comparing the O.D. of the samples to the standard curve.