Human PFKM ELISA Kit

Alternative name
Human ATP-PFK ELISA Kit;Human PFK-M ELISA Kit;Human 6-phosphofructokinase type A ELISA Kit;Human phosphofructo-1-kinase isozyme A ELISA Kit;Human PFK-A ELISA Kit;Human phosphohexokinase ELISA Kit;Human PFKX ELISA Kit;Human GSD7 ELISA Kit;Human PFK-1 ELISA Kit;Human PFK1 ELISA Kit;Human PFKA ELISA Kit;Human PPP1R122 ELISA Kit;Human phosphofructokinase, muscle ELISA Kit;Human ATP-dependent 6-phosphofructokinase, muscle type ELISA Kit;Human 6-phosphofructo-1-kinase ELISA Kit;Human 6-phosphofructokinase, muscle type ELISA Kit;Human phosphofructokinase 1 ELISA Kit;Human phosphofructokinase, polypeptide X ELISA Kit;Human phosphofructokinase-M ELISA Kit;Human protein phosphatase 1, regulatory subunit 122 ELISA Kit;
Catalog
E029067
species
Human
GenePFKM
Standard Curve
Other Species Mouse Pfkm ELISA Kit Bovine PFKM ELISA Kit Canine PFKM ELISA Kit Horse PFKM ELISA Kit Monkey PFKM ELISA Kit Porcine PFKM ELISA Kit Rabbit PFKM ELISA Kit Rat Pfkm ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphofructokinase, Muscle (PFKM). No significant cross-reactivity or interference between Phosphofructokinase, Muscle (PFKM) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.114ng/mL.
Intended UseHuman PFKM ELISA Kit allows for the in vitro quantitative determination of PFKM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphofructokinase, Muscle (PFKM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphofructokinase, Muscle (PFKM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphofructokinase, Muscle (PFKM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphofructokinase, Muscle (PFKM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human ATP-dependent 6-phosphofructokinase, muscle type Protein information
Uniprot ID PFKAM_HUMAN
Uniprot AC P08237; J3KNX3; Q16814; Q16815; Q6ZTT1;
UniGene Hs.75160;
GeneID 5213
KEGG hsa:5213;
Human ATP-dependent 6-phosphofructokinase, muscle type Protein SEQUENCE
SEQUENCE 780 AA; 85183 MW; 769A2C01F97D1122 CRC64;
MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF
VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR
AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA
TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ
RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET
RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME
CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS
HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE
AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE
AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC
TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF
TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG
IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI
FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK
ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV
UCSC uc001rrb.3; human. [P08237-1];

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Human PFKM ELISA Kit