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Human PFKP ELISA Kit

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  • Alternative name

    Human ATP-PFK ELISA Kit;Human PFK-P ELISA Kit;Human 6-phosphofructokinase type C ELISA Kit;Human phosphofructo-1-kinase isozyme C ELISA Kit;Human PFK-C ELISA Kit;Human phosphohexokinase ELISA Kit;Human PFKF ELISA Kit;Human phosphofructokinase, platelet ELISA Kit;Human ATP-dependent 6-phosphofructokinase, platelet type ELISA Kit;Human 6-phosphofructokinase, platelet type ELISA Kit;Human phosphofructokinase 1 ELISA Kit;
  • Catalog
    E029062
  • species
    Human
  • GenePFKP
  • Other Species Mouse Pfkp ELISA KitRabbit PFKP ELISA KitRat Pfkp ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PFKP ELISA Kit allows for the in vitro quantitative determination of PFKP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: PFKP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFKP antibody and an PFKP-HRP conjugate. The assay sample and buffer are incubated together with PFKP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFKP concentration since PFKP from samples and PFKP-HRP conjugate compete for the anti-PFKP antibody binding site. Since the number of sites is limited, as more sites are occupied by PFKP from the sample, fewer sites are left to bind PFKP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFKP concentration in each sample is interpolated from this standard curve.
  • Human ATP-dependent 6-phosphofructokinase, platelet type Protein information
  • Uniprot ID PFKAP_HUMAN
  • Uniprot AC Q01813; B3KS15; Q5VSR7; Q5VSR8;
  • UniGene Hs.26010;
  • GeneID 5214
  • KEGG hsa:5214;
  • Human ATP-dependent 6-phosphofructokinase, platelet type Protein SEQUENCE

  • SEQUENCE 784 AA; 85596 MW; 22522E77E9AF80F6 CRC64;

    MDADDSRAPK GSLRKFLEHL SGAGKAIGVL TSGGDAQGMN AAVRAVVRMG

    IYVGAKVYFI YEGYQGMVDG GSNIAEADWE SVSSILQVGG TIIGSARCQA

    FRTREGRLKA ACNLLQRGIT NLCVIGGDGS LTGANLFRKE WSGLLEELAR

    NGQIDKEAVQ KYAYLNVVGM VGSIDNDFCG TDMTIGTDSA LHRIIEVVDA

    IMTTAQSHQR TFVLEVMGRH CGYLALVSAL ACGADWVFLP ESPPEEGWEE

    QMCVKLSENR ARKKRLNIII VAEGAIDTQN KPITSEKIKE LVVTQLGYDT

    RVTILGHVQR GGTPSAFDRI LASRMGVEAV IALLEATPDT PACVVSLNGN

    HAVRLPLMEC VQMTQDVQKA MDERRFQDAV RLRGRSFAGN LNTYKRLAIK

    LPDDQIPKTN CNVAVINVGA PAAGMNAAVR SAVRVGIADG HRMLAIYDGF

    DGFAKGQIKE IGWTDVGGWT GQGGSILGTK RVLPGKYLEE IATQMRTHSI

    NALLIIGGFE AYLGLLELSA AREKHEEFCV PMVMVPATVS NNVPGSDFSI

    GADTALNTIT DTCDRIKQSA SGTKRRVFII ETMGGYCGYL ANMGGLAAGA

    DAAYIFEEPF DIRDLQSNVE HLTEKMKTTI QRGLVLRNES CSENYTTDFI

    YQLYSEEGKG VFDCRKNVLG HMQQGGAPSP FDRNFGTKIS ARAMEWITAK

    LKEARGRGKK FTTDDSICVL GISKRNVIFQ PVAELKKQTD FEHRIPKEQW

    WLKLRPLMKI LAKYKASYDV SDSGQLEHVQ PWSV

  • UCSC uc001igp.4; human. [Q01813-1];


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