Human PCK2 ELISA Kit

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  • Alternative name

    Human PEPCK-M ELISA Kit;Human PEPCK2 ELISA Kit;Human PEPCK ELISA Kit;Human phosphoenolpyruvate carboxykinase 2, mitochondrial ELISA Kit;Human phosphoenolpyruvate carboxykinase [GTP], mitochondrial ELISA Kit;Human PEP carboxykinase ELISA Kit;Human phosphopyruvate carboxylase ELISA Kit;

  • Catalog
    E029054
  • species
    Human
  • GenePCK2
  • Other Species Mouse Pck2 ELISA KitChicken PCK2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PCK2. No significant cross-reactivity or interference between human PCK2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PCK2 is typically less than 5.88 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman PCK2 ELISA Kit allows for the in vitro quantitative determination of PCK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial Protein information
  • Uniprot ID PCKGM_HUMAN
  • Uniprot AC Q16822; O43253; Q86U01; Q9BV62;
  • UniGene Hs.75812;
  • GeneID 5106
  • KEGG hsa:5106;
  • Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial Protein SEQUENCE
  • SEQUENCE 640 AA; 70730 MW; B8CAC4252EBF8871 CRC64;

    MAALYRPGLR LNWHGLSPLG WPSCRSIQTL RVLSGDLGQL PTGIRDFVEH

    SARLCQPEGI HICDGTEAEN TATLTLLEQQ GLIRKLPKYN NCWLARTDPK

    DVARVESKTV IVTPSQRDTV QLPPGGARGQ LGNWMSPADF QRAVDERFPG

    CMQGRTMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ

    ALGDGDFVKC LHSVGQPLTG QGEPVSQWPC NPEKTLIGHV PDQREIISFG

    SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITS PAGKKRYVAA

    AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGR LRAINPENGF

    FGVAPGTSAT TNPNAMATIQ SNTIFTNVAE TSDGGVYWEG IDQPLPPGVT

    VTSWLGKPWK PGDKEPCAHP NSRFCAPARQ CPIMDPAWEA PEGVPIDAII

    FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KIIMHDPFAM

    RPFFGYNFGH YLEHWLSMEG RKGAQLPRIF HVNWFRRDEA GHFLWPGFGE

    NARVLDWICR RLEGEDSARE TPIGLVPKEG ALDLSGLRAI DTTQLFSLPK

    DFWEQEVRDI RSYLTEQVNQ DLPKEVLAEL EALERRVHKM

  • UCSC uc001wlt.4; human. [Q16822-1];


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