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  • Alternative name

    Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A ELISA KIT; 61 kDa Cam-PDE ELISA KIT; hCam-1PDE1A ELISA KIT; Cam-PDE 1A ELISA KIT

  • Catalog
  • species
  • GenePDE1A
  • Standard CurveHuman PDE1A ELISA Kit
  • Other Species Mouse Pde1a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). No significant cross-reactivity or interference between Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.126ng/mL.
  • Intended UseHuman PDE1A ELISA Kit allows for the in vitro quantitative determination of PDE1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent (PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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