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Human PIP5K1A ELISA Kit

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  • Alternative name

    Human PIP5K1-alpha ELISA Kit;Human ptdIns(4)P-5-kinase 1 alpha ELISA Kit;Human 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha ELISA Kit;Human phosphatidylinositol 4-phosphate 5-kinase type I alpha ELISA Kit;Human PIP5KIalpha ELISA Kit;Human phosphatidylinositol-4-phosphate 5-kinase type 1 alpha ELISA Kit;Human phosphatidylinositol 4-phosphate 5-kinase type-1 alpha ELISA Kit;
  • Catalog
    E028974
  • species
    Human
  • GenePIP5K1A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PIP5K1A. No significant cross-reactivity or interference between Human PIP5K1A and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity31.25 pg/mL (mean of 6 independent assays).
  • Intended UseHuman PIP5K1A ELISA Kit allows for the in vitro quantitative determination of PIP5K1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: For the quantitative detection of Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative PIP5K1A in Human serum, plasma. An antibody specific for PIP5K1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIP5K1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PIP5K1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIP5K1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha Protein information
  • Uniprot ID PI51A_HUMAN
  • Uniprot AC Q99755; A8K4Q0; B4DIN0; Q99754; Q99756;
  • UniGene Hs.655131; Hs.661888;
  • GeneID 8394
  • KEGG hsa:8394;
  • Human Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha Protein SEQUENCE

  • SEQUENCE 562 AA; 62633 MW; A8F7988EB73506A0 CRC64;

    MASASSGPSS SVGFSSFDPA VPSCTLSSAA SGIKRPMASE VLEARQDSYI

    SLVPYASGMP IKKIGHRSVD SSGETTYKKT TSSALKGAIQ LGITHTVGSL

    STKPERDVLM QDFYVVESIF FPSEGSNLTP AHHYNDFRFK TYAPVAFRYF

    RELFGIRPDD YLYSLCSEPL IELCSSGASG SLFYVSSDDE FIIKTVQHKE

    AEFLQKLLPG YYMNLNQNPR TLLPKFYGLY CVQAGGKNIR IVVMNNLLPR

    SVKMHIKYDL KGSTYKRRAS QKEREKPLPT FKDLDFLQDI PDGLFLDADM

    YNALCKTLQR DCLVLQSFKI MDYSLLMSIH NIDHAQREPL SSETQYSVDT

    RRPAPQKALY STAMESIQGE ARRGGTMETD DHMGGIPARN SKGERLLLYI

    GIIDILQSYR FVKKLEHSWK ALVHDGDTVS VHRPGFYAER FQRFMCNTVF

    KKIPLKPSPS KKFRSGSSFS RRAGSSGNSC ITYQPSVSGE HKAQVTTKAE

    VEPGVHLGRP DVLPQTPPLE EISEGSPIPD PSFSPLVGET LQMLTTSTTL

    EKLEVAESEF TH

  • UCSC uc001exi.4; human. [Q99755-1];



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