Human PTEN ELISA Kit

Alternative name
Human mutated in multiple advanced cancers 1 ELISA Kit;Human phosphatase and tensin homolog ELISA Kit;Human MMAC1 ELISA Kit;Human TEP1 ELISA Kit;Human 10q23del ELISA Kit;Human BZS ELISA Kit;Human CWS1 ELISA Kit;Human DEC ELISA Kit;Human GLM2 ELISA Kit;Human MHAM ELISA Kit;Human PTEN1 ELISA Kit;Human phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA Kit;Human MMAC1 phosphatase and tensin homolog deleted on chromosome 10 ELISA Kit;Human mitochondrial PTENalpha ELISA Kit;Human mitochondrial phosphatase and tensin protein alpha ELISA Kit;Human phosphatase and tensin-like protein ELISA Kit;Human phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA Kit;
Catalog
E028957
species
Human
GenePTEN
Other Species Mouse PTEN/MMAC1 ELISA Kit Mouse Pten ELISA Kit Canine PTEN ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman PTEN ELISA Kit allows for the in vitro quantitative determination of PTEN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PTEN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PTEN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PTEN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PTEN can be calculated.
Human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Protein information
Uniprot ID PTEN_HUMAN
Uniprot AC P60484; B2R904; F2YHV0; O00633; O02679; Q6ICT7;
UniGene Hs.500466; Hs.729457;
GeneID 5728
KEGG hsa:5728;
Human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Protein SEQUENCE
SEQUENCE 403 AA; 47166 MW; 75F97C3DD6802BA9 CRC64;
MTAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI
DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL
IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE
ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF
ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC
GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI
DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT
VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHTQI
TKV
UCSC uc001kfb.4; human. [P60484-1];

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Human PTEN ELISA Kit