Human PEMT ELISA Kit

Alternative name
Human PEAMT ELISA Kit;Human PEMT ELISA Kit;Human PEMT2 ELISA Kit;Human phospholipid methyltransferase ELISA Kit;Human PLMT ELISA Kit;Human PEMPT ELISA Kit;Human PNMT ELISA Kit;Human phosphatidylethanolamine N-methyltransferase ELISA Kit;
Catalog
E028905
species
Human
GenePEMT
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PEMT. No significant cross-reactivity or interference between human PEMT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PEMT is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman PEMT ELISA Kit allows for the in vitro quantitative determination of PEMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Phosphatidylethanolamine N-methyltransferase Protein information
Uniprot ID PEMT_HUMAN
Uniprot AC Q9Y6V9;
UniGene Hs.714193;
GeneID 10400
KEGG hsa:10400;
Human Phosphatidylethanolamine N-methyltransferase Protein SEQUENCE
SEQUENCE 199 AA; 22134 MW; 98C07AB54B3B4E6A CRC64;
MTRLLGYVDP LDPSFVAAVI TITFNPLYWN VVARWEHKTR KLSRAFGSPY
LACYSLSVTI LLLNFLRSHC FTQAMLSQPR MESLDTPAAY SLGLALLGLG
VVLVLSSFFA LGFAGTFLGD YFGILKEARV TVFPFNILDN PMYWGSTANY
LGWAIMHASP TGLLLTVLVA LTYIVALLYE EPFTAEIYRQ KASGSHKRS
UCSC uc002grj.3; human. [Q9UBM1-1];

Category

Human PEMT ELISA Kit

Human PEAMT ELISA Kit;Human PEMT ELISA Kit;Human PEMT2 ELISA Kit;Human phospholipid methyltransferase ELISA Kit;Human PLMT ELISA Kit;Human PEMPT ELISA Kit;Human PNMT ELISA Kit;Human phosphatidylethanolamine N-methyltransferase ELISA Kit;