Human PHACTR1 ELISA Kit

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  • Alternative name

    Human KIAA1733 ELISA Kit;Human RPEL1 ELISA Kit;Human RPEL ELISA Kit;Human dJ257A7.2 ELISA Kit;Human phosphatase and actin regulator 1 ELISA Kit;Human RPEL repeat containing 1 ELISA Kit;

  • Catalog
    E028867
  • species
    Human
  • GenePHACTR1
  • Standard CurveHuman PHACTR1 ELISA Kit
  • Other Species Mouse Phactr1 ELISA KitChicken PHACTR1 ELISA KitRat Phactr1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PHACTR1 ELISA Kit allows for the in vitro quantitative determination of PHACTR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: PHACTR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PHACTR1 antibody and an PHACTR1-HRP conjugate. The assay sample and buffer are incubated together with PHACTR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PHACTR1 concentration since PHACTR1 from samples and PHACTR1-HRP conjugate compete for the anti-PHACTR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PHACTR1 from the sample, fewer sites are left to bind PHACTR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PHACTR1 concentration in each sample is interpolated from this standard curve.
  • Human Phosphatase and actin regulator 1 Protein information
  • Uniprot ID PHAR1_HUMAN
  • Uniprot AC Q9C0D0; A8K1V2; Q3MJ93; Q5JSJ2;
  • UniGene Hs.436996;
  • GeneID 221692
  • KEGG hsa:221692;
  • Human Phosphatase and actin regulator 1 Protein SEQUENCE
  • SEQUENCE 580 AA; 66308 MW; 5111973836BBEA39 CRC64;

    MDYPKMDYFL DVESAHRLLD VESAQRFFYS QGAQARRATL LLPPTLMAAS

    SEDDIDRRPI RRVRSKSDTP YLAEARISFN LGAAEEVERL AAMRSDSLVP

    GTHTPPIRRR SKFANLGRIF KPWKWRKKKS EKFKHTSAAL ERKISMRQSR

    EELIKRGVLK EIYDKDGELS ISNEEDSLEN GQSLSSSQLS LPALSEMEPV

    PMPRDPCSYE VLQPSDIMDG PDPGAPVKLP CLPVKLSPPL PPKKVMICMP

    VGGPDLSLVS YTAQKSGQQG VAQHHHTVLP SQIQHQLQYG SHGQHLPSTT

    GSLPMHPSGC RMIDELNKTL AMTMQRLESS EQRVPCSTSY HSSGLHSGDG

    VTKAGPMGLP EIRQVPTVVI ECDDNKENVP HESDYEDSSC LYTREEEEEE

    EDEDDDSSLY TSSLAMKVCR KDSLAIKLSN RPSKRELEEK NILPRQTDEE

    RLELRQQIGT KLTRRLSQRP TAEELEQRNI LKPRNEQEEQ EEKREIKRRL

    TRKLSQRPTV EELRERKILI RFSDYVEVAD AQDYDRRADK PWTRLTAADK

    AAIRKELNEF KSTEMEVHEL SRHLTRFHRP

  • UCSC uc003nag.3; human. [Q9C0D0-1];


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