Human PCNP ELISA Kit;Human PEST-containing nuclear protein ELISA Kit;Human PEST proteolytic signal containing nuclear protein ELISA Kit;Human PEST proteolytic signal-containing nuclear protein ELISA Kit;
Catalog
E028790
species
Human
GenePCNP
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PCNP. No significant cross-reactivity or interference between human PCNP and analogues was observed.
SensitivityThe minimum detectable dose of human PCNP is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman PCNP ELISA Kit allows for the in vitro quantitative determination of PCNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human PEST proteolytic signal-containing nuclear protein Protein information
Uniprot ID
PCNP_HUMAN
Uniprot AC
Q8WW12; B2RBE7; D3DN52; Q53GF3; Q6AI44; Q96CU3; Q9NS81;
UniGene
Hs.732633; Hs.744968;
GeneID
57092
KEGG
hsa:57092;
Human PEST proteolytic signal-containing nuclear protein Protein SEQUENCE