Human PPAR Alpha ELISA Kit

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  • Alternative name

    Peroxisome proliferator-activated receptor alpha ELISA KIT; Nuclear receptor subfamily 1 group C member 1PPARA ELISA KIT; NR1C1 ELISA KIT; PPAR ELISA KIT; PPAR-alpha ELISA KIT

  • Catalog
    E028769
  • species
    Human
  • GenePPAR Alpha
  • Standard CurveHuman PPAR Alpha ELISA Kit
  • Other Species Human PPAR-alpha ELISA KitMouse PPAR-alpha ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PPAR Alpha ELISA Kit allows for the in vitro quantitative determination of PPAR Alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: PPAR-a ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PPAR-a. Standards or samples are then added to the microtiter plate wells and PPAR-a if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PPAR-a present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PPAR-a are added to each well to "sandwich" the PPAR-a immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PPAR-a and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAR-a concentration in each sample is interpolated from this standard curve.



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