Human PPARalpha ELISA Kit

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  • Alternative name

    Peroxisome proliferator-activated receptor alpha ELISA KIT; Nuclear receptor subfamily 1 group C member 1PPARA ELISA KIT; NR1C1 ELISA KIT; PPAR ELISA KIT; PPAR-alpha ELISA KIT

  • Catalog
  • species
  • GenePPARalpha
  • Other Species Mouse PPARalpha ELISA Kit
  • SpecificityNo significant cross-reactivity or interference between Human PPARalpha and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman PPARalpha ELISA Kit allows for the in vitro quantitative determination of PPARalpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PPARalpha concentrations in Human serum, plasma and other body fluids. Using Purified Human PPARalpha antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PPARalpha and PPARalpha antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PPARalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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