Human PPARG ELISA Kit

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  • Alternative name

    Human PPAR-gamma ELISA Kit;Human nuclear receptor subfamily 1 group C member 3 ELISA Kit;Human NR1C3 ELISA Kit;Human CIMT1 ELISA Kit;Human GLM1 ELISA Kit;Human PPARG1 ELISA Kit;Human PPARG2 ELISA Kit;Human PPARgamma ELISA Kit;Human peroxisome proliferator activated receptor gamma ELISA Kit;Human peroxisome proliferator-activated receptor gamma ELISA Kit;Human peroxisome proliferator-activated nuclear receptor gamma variant 1 ELISA Kit;

  • Catalog
    E028746
  • species
    Human
  • GenePPARG
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma (PPARg). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma (PPARg) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseHuman PPARG ELISA Kit allows for the in vitro quantitative determination of PPARG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma (PPARg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma (PPARg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Peroxisome proliferator-activated receptor gamma Protein information
  • Uniprot ID PPARG_HUMAN
  • Uniprot AC Q15179; Q15180; Q15832; Q86U60; Q96J12;
  • UniGene Hs.162646;
  • GeneID 5468
  • KEGG hsa:5468;
  • Human Peroxisome proliferator-activated receptor gamma Protein SEQUENCE
  • SEQUENCE 505 AA; 57620 MW; 3933EFF36A0E4CAF CRC64;

    MGETLGDSPI DPESDSFTDT LSANISQEMT MVDTEMPFWP TNFGISSVDL

    SVMEDHSHSF DIKPFTTVDF SSISTPHYED IPFTRTDPVV ADYKYDLKLQ

    EYQSAIKVEP ASPPYYSEKT QLYNKPHEEP SNSLMAIECR VCGDKASGFH

    YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA

    VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY

    IKSFPLTKAK ARAILTGKTT DKSPFVIYDM NSLMMGEDKI KFKHITPLQE

    QSKEVAIRIF QGCQFRSVEA VQEITEYAKS IPGFVNLDLN DQVTLLKYGV

    HEIIYTMLAS LMNKDGVLIS EGQGFMTREF LKSLRKPFGD FMEPKFEFAV

    KFNALELDDS DLAIFIAVII LSGDRPGLLN VKPIEDIQDN LLQALELQLK

    LNHPESSQLF AKLLQKMTDL RQIVTEHVQL LQVIKKTETD MSLHPLLQEI

    YKDLY

  • UCSC uc003bwr.4; human. [P37231-1];



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