Human PPARD ELISA Kit

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  • Alternative name

    Human PPAR-delta ELISA Kit;Human NUCI ELISA Kit;Human nuclear hormone receptor 1 ELISA Kit;Human NUC1 ELISA Kit;Human nuclear receptor subfamily 1 group C member 2 ELISA Kit;Human peroxisome proliferator-activated receptor beta ELISA Kit;Human PPAR-beta ELISA Kit;Human NR1C2 ELISA Kit;Human PPARB ELISA Kit;Human FAAR ELISA Kit;Human NUCII ELISA Kit;Human peroxisome proliferator activated receptor delta ELISA Kit;Human peroxisome proliferator-activated receptor delta ELISA Kit;Human peroxisome proliferator-activated nuclear receptor beta/delta variant 2 ELISA Kit;

  • Catalog
    E028745
  • species
    Human
  • GenePPARD
  • Standard CurveHuman PPARD ELISA Kit
  • Other Species Mouse Ppard ELISA KitCanine PPARD ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PPARd. No significant cross-reactivity or interference between PPARd and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PPARd is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
  • Intended UseHuman PPARD ELISA Kit allows for the in vitro quantitative determination of PPARD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPARd. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PPARd in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Peroxisome proliferator-activated receptor delta Protein information
  • Uniprot ID PPARD_HUMAN
  • Uniprot AC Q9BUD4;
  • UniGene Hs.696032;
  • GeneID 5467
  • KEGG hsa:5467;
  • Human Peroxisome proliferator-activated receptor delta Protein SEQUENCE
  • SEQUENCE 441 AA; 49903 MW; 94FBB2A4B46521E8 CRC64;

    MEQPQEEAPE VREEEEKEEV AEAEGAPELN GGPQHALPSS SYTDLSRSSS

    PPSLLDQLQM GCDGASCGSL NMECRVCGDK ASGFHYGVHA CEGCKGFFRR

    TIRMKLEYEK CERSCKIQKK NRNKCQYCRF QKCLALGMSH NAIRFGRMPE

    AEKRKLVAGL TANEGSQYNP QVADLKAFSK HIYNAYLKNF NMTKKKARSI

    LTGKASHTAP FVIHDIETLW QAEKGLVWKQ LVNGLPPYKE ISVHVFYRCQ

    CTTVETVREL TEFAKSIPSF SSLFLNDQVT LLKYGVHEAI FAMLASIVNK

    DGLLVANGSG FVTREFLRSL RKPFSDIIEP KFEFAVKFNA LELDDSDLAL

    FIAAIILCGD RPGLMNVPRV EAIQDTILRA LEFHLQANHP DAQYLFPKLL

    QKMADLRQLV TEHAQMMQRI KKTETETSLH PLLQEIYKDM Y

  • UCSC uc003okm.3; human. [Q03181-1];


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