Human PGP ELISA Kit

Alternative name
Human G3PP ELISA Kit;Human aspartate-based ubiquitous Mg(2+)-dependent phosphatase ELISA Kit;Human AUM ELISA Kit;Human phosphoglycolate phosphatase ELISA Kit;Human PGP ELISA Kit;Human PGPase ELISA Kit;Human glycerol-3-phosphate phosphatase ELISA Kit;
Catalog
E028675
species
Human
GenePGP
SpecificityThis assay has high sensitivity and excellent specificity for detection of Permeability Glycoprotein (Pgp). No significant cross-reactivity or interference between Permeability Glycoprotein (Pgp) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.117ng/mL.
Intended UseHuman PGP ELISA Kit allows for the in vitro quantitative determination of PGP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Permeability Glycoprotein (Pgp). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Permeability Glycoprotein (Pgp). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Permeability Glycoprotein (Pgp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Permeability Glycoprotein (Pgp) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glycerol-3-phosphate phosphatase Protein information
Uniprot ID PGP_HUMAN
Uniprot AC A6NDG6;
UniGene Hs.442634;
GeneID 283871
KEGG hsa:283871;
Human Glycerol-3-phosphate phosphatase Protein SEQUENCE
SEQUENCE 321 AA; 34006 MW; 6277550764EAAF91 CRC64;
MAAAEAGGDD ARCVRLSAER AQALLADVDT LLFDCDGVLW RGETAVPGAP
EALRALRARG KRLGFITNNS SKTRAAYAEK LRRLGFGGPA GPGASLEVFG
TAYCTALYLR QRLAGAPAPK AYVLGSPALA AELEAVGVAS VGVGPEPLQG
EGPGDWLHAP LEPDVRAVVV GFDPHFSYMK LTKALRYLQQ PGCLLVGTNM
DNRLPLENGR FIAGTGCLVR AVEMAAQRQA DIIGKPSRFI FDCVSQEYGI
NPERTVMVGD RLDTDILLGA TCGLKTILTL TGVSTLGDVK NNQESDCVSK
KKMVPDFYVD SIADLLPALQ G
UCSC uc002cpk.2; human.;

Category

Human PGP ELISA Kit

Human G3PP ELISA Kit;Human aspartate-based ubiquitous Mg(2+)-dependent phosphatase ELISA Kit;Human AUM ELISA Kit;Human phosphoglycolate phosphatase ELISA Kit;Human PGP ELISA Kit;Human PGPase ELISA Kit;Human glycerol-3-phosphate phosphatase ELISA Kit;