Human PLIN1 ELISA Kit

Alternative name
Human lipid droplet-associated protein ELISA Kit;Human PERI ELISA Kit;Human PLIN ELISA Kit;Human FPLD4 ELISA Kit;Human perilipin 1 ELISA Kit;Human perilipin-1 ELISA Kit;
Catalog
E028634
species
Human
GenePLIN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Perilipin 1 (PLIN1). No significant cross-reactivity or interference between Perilipin 1 (PLIN1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.053ng/mL.
Intended UseHuman PLIN1 ELISA Kit allows for the in vitro quantitative determination of PLIN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perilipin 1 (PLIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perilipin 1 (PLIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Perilipin 1 (PLIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Perilipin 1 (PLIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Perilipin-1 Protein information
Uniprot ID PLIN1_HUMAN
Uniprot AC O60240; Q8N5Y6;
UniGene Hs.103253;
GeneID 5346
KEGG hsa:5346;
Human Perilipin-1 Protein SEQUENCE
SEQUENCE 522 AA; 55990 MW; 394FC14388EB5DCC CRC64;
MAVNKGLTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YTSTKEAHPL
VASVCNAYEK GVQSASSLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE
KIPALQYPPE KIASELKDTI STRLRSARNS ISVPIASTSD KVLGAALAGC
ELAWGVARDT AEFAANTRAG RLASGGADLA LGSIEKVVEY LLPPDKEESA
PAPGHQQAQK SPKAKPSLLS RVGALTNTLS RYTVQTMARA LEQGHTVAMW
IPGVVPLSSL AQWGASVAMQ AVSRRRSEVR VPWLHSLAAA QEEDHEDQTD
TEGEDTEEEE ELETEENKFS EVAALPGPRG LLGGVAHTLQ KTLQTTISAV
TWAPAAVLGM AGRVLHLTPA PAVSSTKGRA MSLSDALKGV TDNVVDTVVH
YVPLPRLSLM EPESEFRDID NPPAEVERRE AERRASGAPS AGPEPAPRLA
QPRRSLRSAQ SPGAPPGPGL EDEVATPAAP RPGFPAVPRE KPKRRVSDSF
FRPSVMEPIL GRTHYSQLRK KS
UCSC uc002boh.4; human.;

Category

Human PLIN1 ELISA Kit

Human lipid droplet-associated protein ELISA Kit;Human PERI ELISA Kit;Human PLIN ELISA Kit;Human FPLD4 ELISA Kit;Human perilipin 1 ELISA Kit;Human perilipin-1 ELISA Kit;