SpecificityThis assay has high sensitivity and excellent specificity for detection of Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4).
No significant cross-reactivity or interference between Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) and analogues was observed.
Intended UseHuman HPF4 ELISA Kit allows for the in vitro quantitative determination of HPF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.