SpecificityThis assay has high sensitivity and excellent specificity for detection of human PIN1. No significant cross-reactivity or interference between human PIN1 and analogues was observed.
SensitivityThe minimum detectable dose of human PIN1 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman PIN1 ELISA Kit allows for the in vitro quantitative determination of PIN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Protein information
Uniprot ID
PIN1_HUMAN
Uniprot AC
Q13526; A8K4V9; Q53X75;
UniGene
Hs.465849;
GeneID
5300
KEGG
hsa:5300;
Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Protein SEQUENCE