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Human PAM ELISA Kit

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  • Alternative name

    Human PAM ELISA Kit;Human PHM ELISA Kit;Human peptidylamidoglycolate lyase ELISA Kit;Human PAL ELISA Kit;Human peptidylglycine alpha-amidating monooxygenase ELISA Kit;Human peptidyl-glycine alpha-amidating monooxygenase ELISA Kit;Human pancreatic peptidylglycine alpha-amidating monooxygenase ELISA Kit;Human peptidyl alpha-amidating enzyme ELISA Kit;Human peptidyl-alpha-hydroxyglycine alpha-amidating lyase ELISA Kit;Human peptidylglycine 2-hydroxylase ELISA Kit;Human peptidylglycine alpha-hydroxylating monooxygenase ELISA Kit;
  • Catalog
    E028593
  • species
    Human
  • GenePAM
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PAM. No significant cross-reactivity or interference between PAM and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PAM is typically less than 13.5pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
  • Intended UseHuman PAM ELISA Kit allows for the in vitro quantitative determination of PAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Protein information
  • Uniprot ID AMD_HUMAN
  • Uniprot AC Q86U53; Q8WVC7; Q9UCG0;
  • UniGene Hs.369430;
  • GeneID 5066
  • KEGG hsa:5066;
  • Human Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Protein SEQUENCE

  • SEQUENCE 973 AA; 108332 MW; 8A089B657F56EE39 CRC64;

    MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV

    PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT

    VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG

    FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY

    LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV

    RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH

    IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP

    VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQGDF YSLLSKLLGE

    REDVVHVHKY NPTEKAESES DLVAEIANVV QKKDLGRSDA REGAEHERGN

    AILVRDRIHK FHRLVSTLRP PESRVFSLQQ PPPGEGTWEP EHTGDFHMEE

    ALDWPGVYLL PGQVSGVALD PKNNLVIFHR GDHVWDGNSF DSKFVYQQIG

    LGPIEEDTIL VIDPNNAAVL QSSGKNLFYL PHGLSIDKDG NYWVTDVALH

    QVFKLDPNNK EGPVLILGRS MQPGSDQNHF CQPTDVAVDP GTGAIYVSDG

    YCNSRIVQFS PSGKFITQWG EESSGSSPLP GQFTVPHSLA LVPLLGQLCV

    ADRENGRIQC FKTDTKEFVR EIKHSSFGRN VFAISYIPGL LFAVNGKPHF

    GDQEPVQGFV MNFSNGEIID IFKPVRKHFD MPHDIVASED GTVYIGDAHT

    NTVWKFTLTE KLEHRSVKKA GIEVQEIKEA EAVVETKMEN KPTSSELQKM

    QEKQKLIKEP GSGVPVVLIT TLLVIPVVVL LAIAIFIRWK KSRAFGDSEH

    KLETSSGRVL GRFRGKGSGG LNLGNFFASR KGYSRKGFDR LSTEGSDQEK

    EDDGSESEEE YSAPLPALAP SSS

  • UCSC uc003kns.4; human. [P19021-1];



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