Intended UseHuman PIN4 ELISA Kit allows for the in vitro quantitative determination of PIN4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description specificalPrinciple of the Assay: PIN4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PIN4 antibody and an PIN4-HRP conjugate. The assay sample and buffer are incubated together with PIN4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIN4 concentration since PIN4 from samples and PIN4-HRP conjugate compete for the anti-PIN4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PIN4 from the sample, fewer sites are left to bind PIN4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIN4 concentration in each sample is interpolated from this standard curve.
Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Protein information
Uniprot ID
PIN4_HUMAN
Uniprot AC
Q6IRW4;
UniGene
Hs.655623;
GeneID
5303
KEGG
hsa:5303;
Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Protein SEQUENCE