Human PIN4 ELISA Kit

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  • Alternative name

    Human parvulin-14 ELISA Kit;Human PAR14 ELISA Kit;Human hPar14 ELISA Kit;Human parvulin-17 ELISA Kit;Human PAR17 ELISA Kit;Human hPar17 ELISA Kit;Human peptidyl-prolyl cis-trans isomerase Pin4 ELISA Kit;Human PPIase PIN4 ELISA Kit;Human peptidyl-prolyl cis/trans isomerase EPVH ELISA Kit;Human hEPVH ELISA Kit;Human rotamase PIN4 ELISA Kit;Human EPVH ELISA Kit;Human peptidylprolyl cis/trans isomerase, NIMA-interacting 4 ELISA Kit;Human peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 ELISA Kit;Human eukaryotic parvulin homolog ELISA Kit;Human parvulin ELISA Kit;Human protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin) ELISA Kit;

  • Catalog
    E028577
  • species
    Human
  • GenePIN4
  • Standard CurveHuman PIN4 ELISA Kit
  • Other Species Mouse Pin4 ELISA KitBovine PIN4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 U/L.
  • Intended UseHuman PIN4 ELISA Kit allows for the in vitro quantitative determination of PIN4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: PIN4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PIN4 antibody and an PIN4-HRP conjugate. The assay sample and buffer are incubated together with PIN4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIN4 concentration since PIN4 from samples and PIN4-HRP conjugate compete for the anti-PIN4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PIN4 from the sample, fewer sites are left to bind PIN4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIN4 concentration in each sample is interpolated from this standard curve.
  • Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Protein information
  • Uniprot ID PIN4_HUMAN
  • Uniprot AC Q6IRW4;
  • UniGene Hs.655623;
  • GeneID 5303
  • KEGG hsa:5303;
  • Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Protein SEQUENCE
  • SEQUENCE 131 AA; 13810 MW; 787C15BDB0701258 CRC64;

    MPPKGKSGSG KAGKGGAASG SDSADKKAQG PKGGGNAVKV RHILCEKHGK

    IMEAMEKLKS GMRFNEVAAQ YSEDKARQGG DLGWMTRGSM VGPFQEAAFA

    LPVSGMDKPV FTDPPVKTKF GYHIIMVEGR K

  • UCSC uc004eam.4; human. [Q9Y237-1];


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