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Human PADI3 ELISA Kit

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  • Alternative name

    Human peptidylarginine deiminase III ELISA Kit;Human protein-arginine deiminase type III ELISA Kit;Human PAD3 ELISA Kit;Human PDI3 ELISA Kit;Human UHS1 ELISA Kit;Human peptidyl arginine deiminase 3 ELISA Kit;Human protein-arginine deiminase type-3 ELISA Kit;Human peptidyl arginine deiminase, type III ELISA Kit;
  • Catalog
    E028567
  • species
    Human
  • GenePADI3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type III (PADI3). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type III (PADI3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.067ng/mL.
  • Intended UseHuman PADI3 ELISA Kit allows for the in vitro quantitative determination of PADI3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type III (PADI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type III (PADI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type III (PADI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type III (PADI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein-arginine deiminase type-3 Protein information
  • Uniprot ID PADI3_HUMAN
  • Uniprot AC Q9ULW8; Q58EY7; Q70SX5;
  • UniGene Hs.149195;
  • GeneID 51702
  • KEGG hsa:51702;
  • Human Protein-arginine deiminase type-3 Protein SEQUENCE

  • SEQUENCE 664 AA; 74743 MW; 90C22A50BF6FD480 CRC64;

    MSLQRIVRVS LEHPTSAVCV AGVETLVDIY GSVPEGTEMF EVYGTPGVDI

    YISPNMERGR ERADTRRWRF DATLEIIVVM NSPSNDLNDS HVQISYHSSH

    EPLPLAYAVL YLTCVDISLD CDLNCEGRQD RNFVDKRQWV WGPSGYGGIL

    LVNCDRDDPS CDVQDNCDQH VHCLQDLEDM SVMVLRTQGP AALFDDHKLV

    LHTSSYDAKR AQVFHICGPE DVCEAYRHVL GQDKVSYEVP RLHGDEERFF

    VEGLSFPDAG FTGLISFHVT LLDDSNEDFS ASPIFTDTVV FRVAPWIMTP

    STLPPLEVYV CRVRNNTCFV DAVAELARKA GCKLTICPQA ENRNDRWIQD

    EMELGYVQAP HKTLPVVFDS PRNGELQDFP YKRILGPDFG YVTREPRDRS

    VSGLDSFGNL EVSPPVVANG KEYPLGRILI GGNLPGSSGR RVTQVVRDFL

    HAQKVQPPVE LFVDWLAVGH VDEFLSFVPA PDGKGFRMLL ASPGACFKLF

    QEKQKCGHGR ALLFQGVVDD EQVKTISINQ VLSNKDLINY NKFVQSCIDW

    NREVLKRELG LAECDIIDIP QLFKTERKKA TAFFPDLVNM LVLGKHLGIP

    KPFGPIINGC CCLEEKVRSL LEPLGLHCTF IDDFTPYHML HGEVHCGTNV

    CRKPFSFKWW NMVP

  • UCSC uc001bai.4; human.;



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