Human PGA3 ELISA Kit

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  • Alternative name

    Human pepsinogen-3 ELISA Kit;Human pepsinogen 3, group I (pepsinogen A) ELISA Kit;Human pepsin A-3 ELISA Kit;Human pepsinogen A3 ELISA Kit;

  • Catalog
    E028510
  • species
    Human
  • GenePGA3
  • Standard CurveHuman PGA3 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural human Pepsin A. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman PGA3 ELISA Kit allows for the in vitro quantitative determination of PGA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Pepsin A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsin A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pepsin A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pepsin A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Pepsin A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Pepsin A-3 Protein information
  • Uniprot ID PEPA3_HUMAN
  • Uniprot AC P0DJD8; A8K749; B2R7D6; B7ZW75; P00790; Q7M4R0; Q8N1E3;
  • UniGene Hs.601055; Hs.661883;
  • GeneID 643834
  • KEGG hsa:643834;
  • Human Pepsin A-3 Protein SEQUENCE
  • SEQUENCE 388 AA; 41976 MW; 5DA16E41FE198582 CRC64;

    MKWLLLLGLV ALSECIMYKV PLIRKKSLRR TLSERGLLKD FLKKHNLNPA

    RKYFPQWKAP TLVDEQPLEN YLDMEYFGTI GIGTPAQDFT VVFDTGSSNL

    WVPSVYCSSL ACTNHNRFNP EDSSTYQSTS ETVSITYGTG SMTGILGYDT

    VQVGGISDTN QIFGLSETEP GSFLYYAPFD GILGLAYPSI SSSGATPVFD

    NIWNQGLVSQ DLFSVYLSAD DQSGSVVIFG GIDSSYYTGS LNWVPVTVEG

    YWQITVDSIT MNGEAIACAE GCQAIVDTGT SLLTGPTSPI ANIQSDIGAS

    ENSDGDMVVS CSAISSLPDI VFTINGVQYP VPPSAYILQS EGSCISGFQG

    MNLPTESGEL WILGDVFIRQ YFTVFDRANN QVGLAPVA

  • UCSC uc001nqx.4; human.;



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