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Human PECAM1 ELISA Kit

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  • Alternative name

    Human PECAM-1 ELISA Kit;Human endoCAM ELISA Kit;Human GPIIA' ELISA Kit;Human PECA1 ELISA Kit;Human CD31 ELISA Kit;Human CD31/EndoCAM ELISA Kit;Human platelet and endothelial cell adhesion molecule 1 ELISA Kit;Human platelet endothelial cell adhesion molecule ELISA Kit;Human CD31 antigen ELISA Kit;Human platelet endothelial cell adhesion molecule-1 ELISA Kit;
  • Catalog
    E028474
  • species
    Human
  • GenePECAM1
  • Standard CurveHuman PECAM1 ELISA Kit
  • Other Species Human PECAM1/CD31 ELISA KitMouse Pecam1 ELISA KitBovine PECAM1 ELISA KitPorcine PECAM1 ELISA KitRat Pecam1 ELISA Kit
  • SpecificityNatural and recombinant human PECAM-1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman PECAM1 ELISA Kit allows for the in vitro quantitative determination of PECAM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PECAM-1 has been precoated onto 96-well plates. Standards(CHO, Q28-K601) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PECAM-1 amount of sample captured in plate. Background: Human platelet/endothelial cell adhesion molecule-1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in theregion q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, isfound in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used inthis kit is the product of gene recombinant expression (extracellular part), consisting of 574 amino acids (Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.
  • Human Platelet endothelial cell adhesion molecule Protein information
  • Uniprot ID PECA1_HUMAN
  • Uniprot AC Q9NPB7; Q9NPG9; Q9NQS9; Q9NQT0; Q9NQT1; Q9NQT2;
  • UniGene Hs.376675; Hs.514412; Hs.722648;
  • GeneID 5175
  • KEGG hsa:5175;
  • Human Platelet endothelial cell adhesion molecule Protein SEQUENCE

  • SEQUENCE 738 AA; 82536 MW; C57BBFA200A407A6 CRC64;

    MQPRWAQGAT MWLGVLLTLL LCSSLEGQEN SFTINSVDMK SLPDWTVQNG

    KNLTLQCFAD VSTTSHVKPQ HQMLFYKDDV LFYNISSMKS TESYFIPEVR

    IYDSGTYKCT VIVNNKEKTT AEYQLLVEGV PSPRVTLDKK EAIQGGIVRV

    NCSVPEEKAP IHFTIEKLEL NEKMVKLKRE KNSRDQNFVI LEFPVEEQDR

    VLSFRCQARI ISGIHMQTSE STKSELVTVT ESFSTPKFHI SPTGMIMEGA

    QLHIKCTIQV THLAQEFPEI IIQKDKAIVA HNRHGNKAVY SVMAMVEHSG

    NYTCKVESSR ISKVSSIVVN ITELFSKPEL ESSFTHLDQG ERLNLSCSIP

    GAPPANFTIQ KEDTIVSQTQ DFTKIASKSD SGTYICTAGI DKVVKKSNTV

    QIVVCEMLSQ PRISYDAQFE VIKGQTIEVR CESISGTLPI SYQLLKTSKV

    LENSTKNSND PAVFKDNPTE DVEYQCVADN CHSHAKMLSE VLRVKVIAPV

    DEVQISILSS KVVESGEDIV LQCAVNEGSG PITYKFYREK EGKPFYQMTS

    NATQAFWTKQ KASKEQEGEY YCTAFNRANH ASSVPRSKIL TVRVILAPWK

    KGLIAVVIIG VIIALLIIAA KCYFLRKAKA KQMPVEMSRP AVPLLNSNNE

    KMSDPNMEAN SHYGHNDDVR NHAMKPINDN KEPLNSDVQY TEVQVSSAES

    HKDLGKKDTE TVYSEVRKAV PDAVESRYSR TEGSLDGT



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