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Catalog
E028473
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species
Human
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GenePECAM-1
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Other Species
Human CD31 / PECAM-1 ELISA KitHuman PECAM 1/CD31 ELISA KitHuman PECAM-1/CD31 ELISA KitMouse CD31 / PECAM-1 ELISA KitMouse PECAM-1 ELISA KitMouse PECAM-1/CD31 ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity Intended Uses For detection of PECAM-1 in human serum, plasma, body fluids, tissue lysates
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Intended UseHuman PECAM-1 ELISA Kit allows for the in vitro quantitative determination of PECAM-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular; Immunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
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Product Description
specificalPrinciple of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PECAM-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PECAM-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PECAM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PECAM-1 can be calculated.
Background: Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31), is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. PECAM1 plays a role as an adhesion receptor in thrombosis, hemostasis, immunity, and inflammatory response, and it is also an important target glycoprotein in drug-induced immune thrombocytopenia.
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