Human PDK4 ELISA Kit

Alternative name
Human Pyruvate dehydrogenase kinase isoform 4 ELISA Kit;Human PDHK4 ELISA Kit;Human pyruvate dehydrogenase kinase 4 ELISA Kit;Human pyruvate dehydrogenase kinase, isozyme 4 ELISA Kit;Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial ELISA Kit;Human [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial ELISA Kit;Human pyruvate dehydrogenase kinase, isoenzyme 4 ELISA Kit;Human pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial ELISA Kit;
Catalog
E028445
species
Human
GenePDK4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PDK4. No significant cross-reactivity or interference between Human PDK4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PDK4 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.938ng/mL
Intended UseHuman PDK4 ELISA Kit allows for the in vitro quantitative determination of PDK4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDK4 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDK4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PDK4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDK4. You can calculate the concentration of Human PDK4 in the samples by comparing the OD of the samples to the standard curve.
Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial Protein information
Uniprot ID PDK4_HUMAN
Uniprot AC Q16654;
UniGene Hs.8364;
GeneID 5166
KEGG hsa:5166;
Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial Protein SEQUENCE
SEQUENCE 411 AA; 46469 MW; C17B78B6057000E9 CRC64;
MKAARFVLRS AGSLNGAGLV PREVEHFSRY SPSPLSMKQL LDFGSENACE
RTSFAFLRQE LPVRLANILK EIDILPTQLV NTSSVQLVKS WYIQSLMDLV
EFHEKSPDDQ KALSDFVDTL IKVRNRHHNV VPTMAQGIIE YKDACTVDPV
TNQNLQYFLD RFYMNRISTR MLMNQHILIF SDSQTGNPSH IGSIDPNCDV
VAVVQDAFEC SRMLCDQYYL SSPELKLTQV NGKFPDQPIH IVYVPSHLHH
MLFELFKNAM RATVEHQENQ PSLTPIEVIV VLGKEDLTIK ISDRGGGVPL
RIIDRLFSYT YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL
YSLSGYGTDA IIYLKALSSE SIEKLPVFNK SAFKHYQMSS EADDWCIPSR
EPKNLAKEVA M
UCSC uc003uoa.4; human.;

Category

Human PDK4 ELISA Kit