Human PDE4D ELISA Kit

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  • Alternative name

    Human DPDE3 ELISA Kit;Human PDE43 ELISA Kit;Human ACRDYS2 ELISA Kit;Human HSPDE4D ELISA Kit;Human PDE4DN2 ELISA Kit;Human STRK1 ELISA Kit;Human phosphodiesterase 4D ELISA Kit;Human cAMP-specific 3',5'-cyclic phosphodiesterase 4D ELISA Kit;Human cAMP-specific phosphodiesterase PDE4D6 ELISA Kit;Human phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) ELISA Kit;Human testicular tissue protein Li 136 ELISA Kit;

  • Catalog
    E028416
  • species
    Human
  • GenePDE4D
  • Other Species Mouse Pde4d ELISA KitRat Pde4d ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PDE4D. No significant cross-reactivity or interference between Human PDE4D and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PDE4D and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseHuman PDE4D ELISA Kit allows for the in vitro quantitative determination of PDE4D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDE4D in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDE4D. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PDE4D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDE4D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDE4D. You can calculate the concentration of Human PDE4D in the samples by comparing the OD of the samples to the standard curve.
  • Human cAMP-specific 3',5'-cyclic phosphodiesterase 4D Protein information
  • Uniprot ID PDE4D_HUMAN
  • Uniprot AC Q8IVD2; Q8IVD3; Q96HL4; Q9HCX7;
  • UniGene Hs.117545;
  • GeneID 5144
  • KEGG hsa:5144;
  • Human cAMP-specific 3',5'-cyclic phosphodiesterase 4D Protein SEQUENCE
  • SEQUENCE 809 AA; 91115 MW; 7A4773DD3A044F57 CRC64;

    MEAEGSSAPA RAGSGEGSDS AGGATLKAPK HLWRHEQHHQ YPLRQPQFRL

    LHPHHHLPPP PPPSPQPQPQ CPLQPPPPPP LPPPPPPPGA ARGRYASSGA

    TGRVRHRGYS DTERYLYCRA MDRTSYAVET GHRPGLKKSR MSWPSSFQGL

    RRFDVDNGTS AGRSPLDPMT SPGSGLILQA NFVHSQRRES FLYRSDSDYD

    LSPKSMSRNS SIASDIHGDD LIVTPFAQVL ASLRTVRNNF AALTNLQDRA

    PSKRSPMCNQ PSINKATITE EAYQKLASET LEELDWCLDQ LETLQTRHSV

    SEMASNKFKR MLNRELTHLS EMSRSGNQVS EFISNTFLDK QHEVEIPSPT

    QKEKEKKKRP MSQISGVKKL MHSSSLTNSS IPRFGVKTEQ EDVLAKELED

    VNKWGLHVFR IAELSGNRPL TVIMHTIFQE RDLLKTFKIP VDTLITYLMT

    LEDHYHADVA YHNNIHAADV VQSTHVLLST PALEAVFTDL EILAAIFASA

    IHDVDHPGVS NQFLINTNSE LALMYNDSSV LENHHLAVGF KLLQEENCDI

    FQNLTKKQRQ SLRKMVIDIV LATDMSKHMN LLADLKTMVE TKKVTSSGVL

    LLDNYSDRIQ VLQNMVHCAD LSNPTKPLQL YRQWTDRIME EFFRQGDRER

    ERGMEISPMC DKHNASVEKS QVGFIDYIVH PLWETWADLV HPDAQDILDT

    LEDNREWYQS TIPQSPSPAP DDPEEGRQGQ TEKFQFELTL EEDGESDTEK

    DSGSQVEEDT SCSDSKTLCT QDSESTEIPL DEQVEEEAVG EEEESQPEAC

    VIDDRSPDT

  • UCSC uc003jrs.4; human. [Q08499-1];


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