Human PDAP1 ELISA Kit

Alternative name
Human PDGF-associated protein ELISA Kit;Human PAP ELISA Kit;Human PDGFA-associated protein 1 ELISA Kit;Human PAP1 ELISA Kit;Human HASPP28 ELISA Kit;Human PDGFA associated protein 1 ELISA Kit;Human 28 kDa heat- and acid-stable phosphoprotein ELISA Kit;
Catalog
E028411
species
Human
GenePDAP1
Other Species Mouse Pdap1 ELISA Kit Rat Pdap1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PDAP1. No significant cross-reactivity or interference between Human PDAP1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PDAP1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman PDAP1 ELISA Kit allows for the in vitro quantitative determination of PDAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDAP1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDAP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PDAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDAP1. You can calculate the concentration of Human PDAP1 in the samples by comparing the OD of the samples to the standard curve.
Human 28 kDa heat- and acid-stable phosphoprotein Protein information
Uniprot ID HAP28_HUMAN
Uniprot AC Q13442; D6W5S5; Q92906;
UniGene Hs.632296;
GeneID 11333
KEGG hsa:11333;
Human 28 kDa heat- and acid-stable phosphoprotein Protein SEQUENCE
SEQUENCE 181 AA; 20630 MW; F97914C7920ABAB3 CRC64;
MPKGGRKGGH KGRARQYTSP EEIDAQLQAE KQKAREEEEQ KEGGDGAAGD
PKKEKKSLDS DESEDEEDDY QQKRKGVEGL IDIENPNRVA QTTKKVTQLD
LDGPKELSRR EREEIEKQKA KERYMKMHLA GKTEQAKADL ARLAIIRKQR
EEAARKKEEE RKAKDDATLS GKRMQSLSLN K
UCSC uc003uqe.5; human.;

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Category

Human PDAP1 ELISA Kit

Human PDGF-associated protein ELISA Kit;Human PAP ELISA Kit;Human PDGFA-associated protein 1 ELISA Kit;Human PAP1 ELISA Kit;Human HASPP28 ELISA Kit;Human PDGFA associated protein 1 ELISA Kit;Human 28 kDa heat- and acid-stable phosphoprotein ELISA Kit;