Human CD274 ELISA Kit

Alternative name
Human PD-L1 ELISA Kit;Human PDCD1 ligand 1 ELISA Kit;Human Programmed death ligand 1 ELISA Kit;Human B7 homolog 1 ELISA Kit;Human B7-H1 ELISA Kit;Human B7H1 ELISA Kit;Human PDCD1L1 ELISA Kit;Human PDCD1LG1 ELISA Kit;Human PDL1 ELISA Kit;Human B7-H ELISA Kit;Human CD274 molecule ELISA Kit;Human programmed cell death 1 ligand 1 ELISA Kit;Human CD274 antigen ELISA Kit;
Catalog
E028410
species
Human
GeneCD274
SpecificityNatural and recombinant human PD-L1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human PD-L1 in cell culture supernates, serum and plas
Intended UseHuman CD274 ELISA Kit allows for the in vitro quantitative determination of CD274 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human PD-L1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PD-L1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: F19-R238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PD-L1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PD-L1 amount of sample captured in plate. Background: Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.
Human Programmed cell death 1 ligand 1 Protein information
Uniprot ID PD1L1_HUMAN
Uniprot AC Q9NZQ7; B2RBA2; B4DU27; Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5;
UniGene Hs.521989;
GeneID 29126
KEGG hsa:29126;
Human Programmed cell death 1 ligand 1 Protein SEQUENCE
SEQUENCE 290 AA; 33275 MW; FE957086E62A31A8 CRC64;
MRIFAVFIFM TYWHLLNAFT VTVPKDLYVV EYGSNMTIEC KFPVEKQLDL
AALIVYWEME DKNIIQFVHG EEDLKVQHSS YRQRARLLKD QLSLGNAALQ
ITDVKLQDAG VYRCMISYGG ADYKRITVKV NAPYNKINQR ILVVDPVTSE
HELTCQAEGY PKAEVIWTSS DHQVLSGKTT TTNSKREEKL FNVTSTLRIN
TTTNEIFYCT FRRLDPEENH TAELVIPELP LAHPPNERTH LVILGAILLC
LGVALTFIFR LRKGRMMDVK KCGIQDTNSK KQSDTHLEET
UCSC uc003zje.4; human. [Q9NZQ7-1];

Human CD274 ELISA Kit

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