Human PCSK9 ELISA Kit

Alternative name
Human Neural apoptosis-regulated convertase 1 ELISA Kit;Human NARC-1 ELISA Kit;Human Proprotein convertase 9 ELISA Kit;Human PC9 ELISA Kit;Human subtilisin/kexin-like protease PC9 ELISA Kit;Human NARC1 ELISA Kit;Human FH3 ELISA Kit;Human HCHOLA3 ELISA Kit;Human LDLCQ1 ELISA Kit;Human proprotein convertase subtilisin/kexin type 9 ELISA Kit;Human convertase subtilisin/kexin type 9 preproprotein ELISA Kit;Human neural apoptosis regulated convertase 1 ELISA Kit;
Catalog
E028403
species
Human
GenePCSK9
SpecificityNatural and recombinant human PCSK9
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman PCSK9 ELISA Kit allows for the in vitro quantitative determination of PCSK9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human PCSK9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PCSK9 has been precoated onto 96-well plates. Standards(NSO, S153-Q692) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PCSK9 amount of sample captured in plate. Background: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins (LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Human Proprotein convertase subtilisin/kexin type 9 Protein information
Uniprot ID PCSK9_HUMAN
Uniprot AC Q8NBP7; A8T640; C0JYY9; Q5PSM5; Q5SZQ2;
UniGene Hs.18844;
GeneID 255738
KEGG hsa:255738;
Human Proprotein convertase subtilisin/kexin type 9 Protein SEQUENCE
SEQUENCE 692 AA; 74286 MW; 9BCB9418B90AEE23 CRC64;
MGTVSSRRSW WPLPLLLLLL LLLGPAGARA QEDEDGDYEE LVLALRSEED
GLAEAPEHGT TATFHRCAKD PWRLPGTYVV VLKEETHLSQ SERTARRLQA
QAARRGYLTK ILHVFHGLLP GFLVKMSGDL LELALKLPHV DYIEEDSSVF
AQSIPWNLER ITPPRYRADE YQPPDGGSLV EVYLLDTSIQ SDHREIEGRV
MVTDFENVPE EDGTRFHRQA SKCDSHGTHL AGVVSGRDAG VAKGASMRSL
RVLNCQGKGT VSGTLIGLEF IRKSQLVQPV GPLVVLLPLA GGYSRVLNAA
CQRLARAGVV LVTAAGNFRD DACLYSPASA PEVITVGATN AQDQPVTLGT
LGTNFGRCVD LFAPGEDIIG ASSDCSTCFV SQSGTSQAAA HVAGIAAMML
SAEPELTLAE LRQRLIHFSA KDVINEAWFP EDQRVLTPNL VAALPPSTHG
AGWQLFCRTV WSAHSGPTRM ATAVARCAPD EELLSCSSFS RSGKRRGERM
EAQGGKLVCR AHNAFGGEGV YAIARCCLLP QANCSVHTAP PAEASMGTRV
HCHQQGHVLT GCSSHWEVED LGTHKPPVLR PRGQPNQCVG HREASIHASC
CHAPGLECKV KEHGIPAPQE QVTVACEEGW TLTGCSALPG TSHVLGAYAV
DNTCVVRSRD VSTTGSTSEG AVTAVAICCR SRHLAQASQE LQ
UCSC uc001cyf.3; human. [Q8NBP7-1];

Category

Human PCSK9 ELISA Kit