Human PAF ELISA Kit

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  • Catalog
    E028398
  • species
    Human
  • GenePAF
  • Standard CurveHuman PAF ELISA Kit
  • Other Species Human lyso-PAF ELISA KitHuman PAF AH ELISA KitMouse lyso-PAF ELISA KitMouse PAF-AH ELISA KitMouse PAF ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PCNA Associated Factor 15kDa (PAF). No significant cross-reactivity or interference between PCNA Associated Factor 15kDa (PAF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseHuman PAF ELISA Kit allows for the in vitro quantitative determination of PAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA Associated Factor 15kDa (PAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PCNA Associated Factor 15kDa (PAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCNA Associated Factor 15kDa (PAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCNA Associated Factor 15kDa (PAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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