Intended UseHuman Anti-GM1 ELISA Kit allows for the in vitro quantitative determination of Anti-GM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human Anti-GM1. Standards or samples are then added to the appropriate micro ELISA plate wells and combined to the specific antigen. Then a biotinylated detection antigen specific for Human Anti-GM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Anti-GM1, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color turn yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Anti-GM1. You can calculate the concentration of Human Anti-GM1 in the samples by comparing the OD of the samples to the standard curve.