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Human PARP4 ELISA Kit

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  • Alternative name

    Human PARP-4 ELISA Kit;Human 193 kDa vault protein ELISA Kit;Human ADP-ribosyltransferase diphtheria toxin-like 4 ELISA Kit;Human ARTD4 ELISA Kit;Human PARP-related/IalphaI-related H5/proline-rich ELISA Kit;Human PH5P ELISA Kit;Human vault poly(ADP-ribose) polymerase ELISA Kit;Human VPARP ELISA Kit;Human ADPRTL1 ELISA Kit;Human KIAA0177 ELISA Kit;Human PARPL ELISA Kit;Human VAULT3 ELISA Kit;Human VWA5C ELISA Kit;Human p193 ELISA Kit;Human poly(ADP-ribose) polymerase family member 4 ELISA Kit;Human poly [ADP-ribose] polymerase 4 ELISA Kit;Human ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1 ELISA Kit;Human H5 proline-rich ELISA Kit;Human I-alpha-I-related ELISA Kit;Human PARP-related ELISA Kit;Human poly(ADP-ribose) synthetase ELISA Kit;Human poly(ADP-ribosyl)transferase-like 1 ELISA Kit;Human vault protein, 193-kDa ELISA Kit;Human von Willebrand factor A domain containing 5C ELISA Kit;
  • Catalog
    E028341
  • species
    Human
  • GenePARP4
  • SpecificityThis kit recognizes natural and recombinant Human PARP4. No significant cross-reactivity or interference between Human PARP4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human PARP4 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman PARP4 ELISA Kit allows for the in vitro quantitative determination of PARP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Priciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PARP4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PARP4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PARP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PARP4. You can calculate the concentration of PARP4 in the samples by comparing the OD of the samples to the standard curve.
  • Human Poly [ADP-ribose] polymerase 4 Protein information
  • Uniprot ID PARP4_HUMAN
  • Uniprot AC Q9UKK3; O75903; Q14682; Q5QNZ9; Q9H1M6;
  • UniGene Hs.744855;
  • GeneID 143
  • KEGG hsa:143;
  • Human Poly [ADP-ribose] polymerase 4 Protein SEQUENCE

  • SEQUENCE 1724 AA; 192595 MW; DCA1DD4C001EA22F CRC64;

    MVMGIFANCI FCLKVKYLPQ QQKKKLQTDI KENGGKFSFS LNPQCTHIIL

    DNADVLSQYQ LNSIQKNHVH IANPDFIWKS IREKRLLDVK NYDPYKPLDI

    TPPPDQKASS SEVKTEGLCP DSATEEEDTV ELTEFGMQNV EIPHLPQDFE

    VAKYNTLEKV GMEGGQEAVV VELQCSRDSR DCPFLISSHF LLDDGMETRR

    QFAIKKTSED ASEYFENYIE ELKKQGFLLR EHFTPEATQL ASEQLQALLL

    EEVMNSSTLS QEVSDLVEMI WAEALGHLEH MLLKPVNRIS LNDVSKAEGI

    LLLVKAALKN GETAEQLQKM MTEFYRLIPH KGTMPKEVNL GLLAKKADLC

    QLIRDMVNVC ETNLSKPNPP SLAKYRALRC KIEHVEQNTE EFLRVRKEVL

    QNHHSKSPVD VLQIFRVGRV NETTEFLSKL GNVRPLLHGS PVQNIVGILC

    RGLLLPKVVE DRGVQRTDVG NLGSGIYFSD SLSTSIKYSH PGETDGTRLL

    LICDVALGKC MDLHEKDFSL TEAPPGYDSV HGVSQTASVT TDFEDDEFVV

    YKTNQVKMKY IIKFSMPGDQ IKDFHPSDHT ELEEYRPEFS NFSKVEDYQL

    PDAKTSSSTK AGLQDASGNL VPLEDVHIKG RIIDTVAQVI VFQTYTNKSH

    VPIEAKYIFP LDDKAAVCGF EAFINGKHIV GEIKEKEEAQ QEYLEAVTQG

    HGAYLMSQDA PDVFTVSVGN LPPKAKVLIK ITYITELSIL GTVGVFFMPA

    TVAPWQQDKA LNENLQDTVE KICIKEIGTK QSFSLTMSIE MPYVIEFIFS

    DTHELKQKRT DCKAVISTME GSSLDSSGFS LHIGLSAAYL PRMWVEKHPE

    KESEACMLVF QPDLDVDLPD LASESEVIIC LDCSSSMEGV TFLQAKQIAL

    HALSLVGEKQ KVNIIQFGTG YKELFSYPKH ITSNTMAAEF IMSATPTMGN

    TDFWKTLRYL SLLYPARGSR NILLVSDGHL QDESLTLQLV KRSRPHTRLF

    ACGIGSTANR HVLRILSQCG AGVFEYFNAK SKHSWRKQIE DQMTRLCSPS

    CHSVSVKWQQ LNPDVPEALQ APAQVPSLFL NDRLLVYGFI PHCTQATLCA

    LIQEKEFRTM VSTTELQKTT GTMIHKLAAR ALIRDYEDGI LHENETSHEM

    KKQTLKSLII KLSKENSLIT QFTSFVAVEK RDENESPFPD IPKVSELIAK

    EDVDFLPYMS WQGEPQEAVR NQSLLASSEW PELRLSKRKH RKIPFSKRKM

    ELSQPEVSED FEEDGLGVLP AFTSNLERGG VEKLLDLSWT ESCKPTATEP

    LFKKVSPWET STSSFFPILA PAVGSYLPPT ARAHSPASLS FASYRQVASF

    GSAAPPRQFD ASQFSQGPVP GTCADWIPQS ASCPTGPPQN PPSSPYCGIV

    FSGSSLSSAQ SAPLQHPGGF TTRPSAGTFP ELDSPQLHFS LPTDPDPIRG

    FGSYHPSASS PFHFQPSAAS LTANLRLPMA SALPEALCSQ SRTTPVDLCL

    LEESVGSLEG SRCPVFAFQS SDTESDELSE VLQDSCFLQI KCDTKDDSIL

    CFLEVKEEDE IVCIQHWQDA VPWTELLSLQ TEDGFWKLTP ELGLILNLNT

    NGLHSFLKQK GIQSLGVKGR ECLLDLIATM LVLQFIRTRL EKEGIVFKSL

    MKMDDASISR NIPWAFEAIK QASEWVRRTE GQYPSICPRL ELGNDWDSAT

    KQLLGLQPIS TVSPLHRVLH YSQG

  • UCSC uc001upl.4; human.;



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