Human PTH1 34 ELISA Kit

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  • Alternative name

    Peptidyl-tRNA hydrolase ELISA KIT; pth ELISA KIT; Sca_0152 ELISA KIT; PTH ELISA KIT

  • Catalog
    E028306
  • species
    Human
  • GenePTH1 34
  • Standard CurveHuman PTH1 34 ELISA Kit
  • Other Species Mouse PTH1-34 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTH1-34. No significant cross-reactivity or interference between PTH1-34 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTH1-34 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PTH1 34 ELISA Kit allows for the in vitro quantitative determination of PTH1 34 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: PTH1-34 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PTH1-34. Standards or samples are then added to the microtiter plate wells and PTH1-34 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PTH1-34 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PTH1-34 are added to each well to "sandwich" the PTH1-34 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PTH1-34 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTH1-34 concentration in each sample is interpolated from this standard curve.


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